Fluorescent-Based Thermal Sensing in Lipid Membranes
Thermal mapping in biological membranes could unlock and help us understand many chemical and physical processes that do not only pertain to localized membrane phenomena but also extend to many other intra- and extracellular pathways. In this manuscript, we report the development of a ratiometric th...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 36(2020), 5 vom: 11. Feb., Seite 1221-1226 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2020
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine Carbocyanines Fluorescent Dyes Liposomes Poly(2,5-methoxy-propyloxy sulfonate phenylene vinylene) Polyelectrolytes Polyvinyls 1,2-Dipalmitoylphosphatidylcholine |
| Zusammenfassung: | Thermal mapping in biological membranes could unlock and help us understand many chemical and physical processes that do not only pertain to localized membrane phenomena but also extend to many other intra- and extracellular pathways. In this manuscript, we report the development of a ratiometric thermal fluorescent probe based on the Förster resonance energy transfer between a lipid-embedded conjugated polyelectrolyte and a lyophilic acceptor dye. We showed that the Förster resonance energy transfer (FRET) pair is sensitive within the relevant physiological temperature window (20.0-50.0 °C). The signal was also shielded from an external pH and stable when cycled multiple times. The probe was also sensitive to the membrane composition and could, therefore, be further developed to probe the membrane composition and viscosity |
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| Beschreibung: | Date Completed 12.04.2021 Date Revised 12.04.2021 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.9b03128 |