Kinase activity-tagged western blotting assay

Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS-PAGE and transfer onto polyvinylidene fluoride m...

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Publié dans:	BioTechniques. - 1991. - 68(2020), 4 vom: 07. Apr., Seite 211-213
Auteur principal:	Eto, Masumi (Auteur)
Autres auteurs:	Katsuki, Shuichi, Tanaka, Yoshinori, Takeya, Kosuke
Format:	Article en ligne
Langue:	English
Publié:	2020
Accès à la collection:	BioTechniques
Sujets:	Journal Article Research Support, Non-U.S. Gov't cell signaling laboratory safety nonradioactive assay phosphorylation post-transcriptional modification protein kinases western blotting Proteins plus... Protein Kinases EC 2.7.-