Nuclease and ribonuclease activities in response to salt stress : Identification of PvRNS3, a T2/S-like ribonuclease induced in common bean radicles by salt stress

Copyright © 2019 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 147(2020) vom: 01. Feb., Seite 235-241
Auteur principal: Diaz-Baena, Mercedes (Auteur)
Autres auteurs: Galvez-Valdivieso, Gregorio, Delgado-Garcia, Elena, Pineda, Manuel, Piedras, Pedro
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Nutrient recycling Phaseolus vulgaris Ribonuclease Salt stress RNS3 ribonuclease EC 3.1.- Ribonucleases
Description
Résumé:Copyright © 2019 Elsevier Masson SAS. All rights reserved.
The increase in soil salinization due to global climate change could cause large losses in crop productivity affecting, among other biological processes, to germination and seedling development. We have studied how salt stress affects nucleic acid degrading activities in radicles of common bean during seedling development. In radicles of common bean, a main nuclease of 37 kDa and two ribonucleases of 17 and 19 kDa were detected. Saline stress did not alter these three activities but induced a new ribonuclease of 16 kDa. All three ribonucleases are acidic enzymes that were inhibited by Zn. The 16 and 17 kDa ribonucleases are inhibited by guanilates. In the genome of common bean, we have identified 13 genes belonging to the T2 ribonuclease family and that are grouped in the 3 classes of T2 ribonucleases. The analysis of the expression of the 3 genes belonging to Class I (PvRNS1 to 3) and the unique gene from Class II (PvRNS4) in radicles showed that PvRNS3 is highly induced under salt stress
Description:Date Completed 27.04.2020
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2019.12.016