Ionomic and metabolomic analyses reveal the resistance response mechanism to saline-alkali stress in Malus halliana seedlings

Copyright © 2019 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 147(2020) vom: 01. Feb., Seite 77-90
Auteur principal: Jia, Xumei (Auteur)
Autres auteurs: Zhu, Yanfang, Zhang, Rui, Zhu, Zulei, Zhao, Tong, Cheng, Li, Gao, Liyang, Liu, Bing, Zhang, Xiayi, Wang, Yanxiu
Format: Article en ligne
Langue:English
Publié: 2020
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Ion homeostasis Metabolites Na(+) detoxification Saline-alkali stress Scavenging of ROS Alkalies
Description
Résumé:Copyright © 2019 Elsevier Masson SAS. All rights reserved.
Saline-alkali stress is a major abiotic stress limiting plant growth. The selection of saline-alkali-tolerant rootstock is an effective strategy to reduce salinization-alkalization influence in apple production. M. halliana is a highly saline-alkali-resistant apple rootstock in northwestern China. However, few metabolic response studies have been conducted on this species. In plants under saline-alkali stress, the uptake of K, Mg and Zn in M. halliana leaves were inhibited, whereas the absorption of Fe2+, Cu2+ or Mn2+ were increased. Metabolic analysis revealed 140 differentially expressed metabolites, which were mainly involved in alkaloid biosynthesis, phenylalanine biosynthesis, ATP-binding cassette (ABC) transporters, and mineral absorption. Especially, the expression of sucrose, amino acids, alkaloids, flavonoids and carotenoids were significantly upregulated under saline-alkali stress. qRT-PCR analysis demonstrated that NHX8 and ZTP1 involved in Na+ and Fe2+ transport were upregulated, while AKT1, MRS2-4 and ZTP29 involved in K+, Mg2+ and Zn2+ transport were downregulated, respectively. ANT, ATP2A, CALM and SOS2 are involved in Ca2+ signal transduction, and ABCB1, ABCC10 and NatA are key transporters that maintain ionic homeostasis. M. halliana regulates Na+/K+ homeostasis by mediating Ca2+ signalling and ABC transporters. The accumulation of metabolites contributes to improving the saline-alkali resistance of M. halliana because of the scavenging of ROS. An increase in pheophorbide a content in porphyrin and chlorophyll metabolism leads to leaf senescence in M. halliana leaves, which contributes to a reduction in stress-induced injury. These findings provide important insights into the saline-alkali tolerance mechanism in apple, which also provides an important starting point for future research
Description:Date Completed 27.04.2020
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2019.12.001