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231225s2020 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2019-0127
|2 doi
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|a pubmed24n1014.xml
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|a DE-627
|b ger
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Kondo, Tomo
|e verfasserin
|4 aut
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| 245 |
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|a An improved molecular tool for screening bacterial colonies using GFP expression enhanced by a Dictyostelium sequence
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.12.2020
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|a Date Revised 14.12.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited. Recently, we reported that translational enhancement by a Dictyostelium gene sequence (TED) boosted protein expression even without an expression inducer in Escherichia coli. Here, we demonstrate a generally applicable molecular tool using the expression of green fluorescent protein enhanced by TED. By inserting a module related to TED into the cloning site in advance, we effectively screened E. coli colonies harboring the desired plasmid functions in a prokaryote (Magnetospirillum gryphiswaldense) or eukaryote (Dictyostelium discoideum). Thus, our system represents a user-friendly technique for cloning
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|a Evaluation Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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4 |
|a Dictyostelium
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| 650 |
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4 |
|a E. coli
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| 650 |
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|a GFP
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| 650 |
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4 |
|a Magnetospirillum
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| 650 |
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4 |
|a molecular cloning
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| 650 |
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4 |
|a screening
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 700 |
1 |
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|a Yumura, Shigehiko
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 68(2020), 2 vom: 20. Feb., Seite 91-95
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
1 |
8 |
|g volume:68
|g year:2020
|g number:2
|g day:20
|g month:02
|g pages:91-95
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.2144/btn-2019-0127
|3 Volltext
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|d 68
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