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20231225114523.0 |
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cr uuu---uuuuu |
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231225s2019 xx |||||o 00| ||eng c |
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7 |
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|a 10.1111/vcp.12812
|2 doi
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|a pubmed24n1014.xml
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|a (DE-627)NLM304219282
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|a (NLM)31820477
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Bridges, Cory S
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Analytical validation of an enzyme-linked immunosorbent assay for the quantification of S100A12 in the serum and feces of cats
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 02.06.2020
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|a Date Revised 02.06.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2019 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Measuring S100A12 concentrations in serum and feces is a sensitive and specific marker of inflammation, such as seen with chronic gastrointestinal inflammation in people and dogs. Biomarkers of inflammation in cats are currently lacking
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|a OBJECTIVES: We aimed to analytically cross-validate the canine S100A12-ELISA for the measurement of S100A12 in feline specimens
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|a METHODS: The ELISA was analytically validated by assessing dilutional linearity, spiking/recovery, intra- and inter-assay variability. Reference intervals for serum and fecal feline S100A12 concentrations were calculated using samples from healthy cats, and the short-term biological variation of fecal S100A12 was assessed
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|a RESULTS: Observed-to-expected ratios (O/E) for serial dilutions of serum and fecal extracts ranged from 91%-159% (mean, 120%) and 100%-128% (mean, 114%), and for the spiking/recovery method ranged from 106%-263% (mean, 154%) and 52%-171% (mean, 112%). Intra- and inter-assay CV% for serum were ≤5.6% and ≤14.0%, and for fecal extracts were ≤3.8% and ≤19.1%, repsectively. RIs for feline serum and fecal S100A12 concentrations were <43 µg/L and < 20 ng/g, respectively. A mild short-term biologic variation, but large individuality were detected when measuring fecal S100A12 concentrations in healthy cats
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|a CONCLUSIONS: The canine S100A12-ELISA is accurate, reproducible, and sufficiently linear and precise for the measurement of S100A12 in feline serum and fecal samples. The use of this assay is a reasonable option for the measurement of S100A12 concentrations in feline specimens and provides a basis for the further evaluation of S100A12 in cats with gastrointestinal disease. Using a population-based RI for fecal feline S100A12 appears to be of limited value
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4 |
|a Journal Article
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| 650 |
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4 |
|a Validation Study
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| 650 |
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4 |
|a Calgranulin C
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| 650 |
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4 |
|a chronic enteropathy
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| 650 |
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4 |
|a feline
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| 650 |
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4 |
|a immunoassay
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| 650 |
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7 |
|a Biomarkers
|2 NLM
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| 650 |
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|a S100A12 Protein
|2 NLM
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| 700 |
1 |
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|a Grützner, Niels
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Suchodolski, Jan S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Steiner, Jörg M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Heilmann, Romy M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 48(2019), 4 vom: 20. Dez., Seite 754-761
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
8 |
|g volume:48
|g year:2019
|g number:4
|g day:20
|g month:12
|g pages:754-761
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/vcp.12812
|3 Volltext
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|d 48
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|e 4
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|c 12
|h 754-761
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