Methodology for the at-home collection of urine samples for prostate cancer detection

Methodology for the at-home collection of urine samples for prostate cancer detection

Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secre...

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Veröffentlicht in:BioTechniques. - 1988. - 68(2020), 2 vom: 01. Feb., Seite 65-71
1. Verfasser: Webb, Martyn (VerfasserIn)
Weitere Verfasser: Manley, Kate, Olivan, Mireia, Guldvik, Ingrid, Palczynska, Malgorzata, Hurst, Rachel, Connell, Shea P, Mills, Ian G, Brewer, Daniel S, Mills, Robert, Cooper, Colin S, Clark, Jeremy
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Controlled Clinical Trial Journal Article Research Support, Non-U.S. Gov't biomarker cancer diagnosis home-screening prognosis prostate urine mehr... RNA 63231-63-0
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520 |a Urine from patients with prostate cancer (PCa) contains gene transcripts that have been used for PCa diagnosis and prognosis. Historically, patient urine samples have been collected after a digital rectal examination of the prostate, which was thought necessary to boost the levels of prostatic secretions in the urine. We herein describe methodology that allows urine to be collected by patients at home and then posted to a laboratory for analysis. RNA yields and quality were comparable to those for post digital rectal examination urine, and there was improved sensitivity for the detection of TMPRSS2:ERG transcripts by RT-PCR. The At-Home collection protocol has opened up the potential to perform large-scale PCa studies without the inconvenience, cost, discomfort and expense of patients having to visit the clinic 
650 4 |a Controlled Clinical Trial 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a biomarker 
650 4 |a cancer 
650 4 |a diagnosis 
650 4 |a home-screening 
650 4 |a prognosis 
650 4 |a prostate 
650 4 |a urine 
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700 1 |a Manley, Kate  |e verfasserin  |4 aut 
700 1 |a Olivan, Mireia  |e verfasserin  |4 aut 
700 1 |a Guldvik, Ingrid  |e verfasserin  |4 aut 
700 1 |a Palczynska, Malgorzata  |e verfasserin  |4 aut 
700 1 |a Hurst, Rachel  |e verfasserin  |4 aut 
700 1 |a Connell, Shea P  |e verfasserin  |4 aut 
700 1 |a Mills, Ian G  |e verfasserin  |4 aut 
700 1 |a Brewer, Daniel S  |e verfasserin  |4 aut 
700 1 |a Mills, Robert  |e verfasserin  |4 aut 
700 1 |a Cooper, Colin S  |e verfasserin  |4 aut 
700 1 |a Clark, Jeremy  |e verfasserin  |4 aut 
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