Thermosensitive Hydrogel Based on PEO-PPO-PEO Poloxamers for a Controlled In Situ Release of Recombinant Adeno-Associated Viral Vectors for Effective Gene Therapy of Cartilage Defects

Thermosensitive Hydrogel Based on PEO-PPO-PEO Poloxamers for a Controlled In Situ Release of Recombinant Adeno-Associated Viral Vectors for Effective Gene Therapy of Cartilage Defects

© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 32(2020), 2 vom: 20. Jan., Seite e1906508
1. Verfasser: Madry, Henning (VerfasserIn)
Weitere Verfasser: Gao, Liang, Rey-Rico, Ana, Venkatesan, Jagadeesh K, Müller-Brandt, Kathrin, Cai, Xiaoyu, Goebel, Lars, Schmitt, Gertrud, Speicher-Mentges, Susanne, Zurakowski, David, Menger, Michael D, Laschke, Matthias W, Cucchiarini, Magali
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article biomaterial-guided therapy cartilage defects gene therapy rAAV tissue engineering Drug Carriers Hydrogels PEO-PPO-PEO Propylene Glycols mehr... Poloxamer 106392-12-5 Polyethylene Glycols 3WJQ0SDW1A
LEADER 01000naa a22002652 4500
001 NLM30366214X
003 DE-627
005 20231225113314.0
007 cr uuu---uuuuu
008 231225s2020 xx |||||o 00| ||eng c
024 7 |a 10.1002/adma.201906508  |2 doi 
028 5 2 |a pubmed24n1012.xml 
035 |a (DE-627)NLM30366214X 
035 |a (NLM)31763733 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Madry, Henning  |e verfasserin  |4 aut 
245 1 0 |a Thermosensitive Hydrogel Based on PEO-PPO-PEO Poloxamers for a Controlled In Situ Release of Recombinant Adeno-Associated Viral Vectors for Effective Gene Therapy of Cartilage Defects 
264 1 |c 2020 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 16.11.2020 
500 |a Date Revised 16.11.2020 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. 
520 |a Advanced biomaterial-guided delivery of gene vectors is an emerging and highly attractive therapeutic solution for targeted articular cartilage repair, allowing for a controlled and minimally invasive delivery of gene vectors in a spatiotemporally precise manner, reducing intra-articular vector spread and possible loss of the therapeutic gene product. As far as it is known, the very first successful in vivo application of such a biomaterial-guided delivery of a potent gene vector in an orthotopic large animal model of cartilage damage is reported here. In detail, an injectable and thermosensitive hydrogel based on poly(ethylene oxide) (PEO)-poly(propylene oxide) (PPO)-PEO poloxamers, capable of controlled release of a therapeutic recombinant adeno-associated virus (rAAV) vector overexpressing the chondrogenic sox9 transcription factor in full-thickness chondral defects, is applied in a clinically relevant minipig model in vivo. These comprehensive analyses of the entire osteochondral unit with multiple standardized evaluation methods indicate that rAAV-FLAG-hsox9/PEO-PPO-PEO hydrogel-augmented microfracture significantly improves cartilage repair with a collagen fiber orientation more similar to the normal cartilage and protects the subchondral bone plate from early bone loss 
650 4 |a Journal Article 
650 4 |a biomaterial-guided therapy 
650 4 |a cartilage defects 
650 4 |a gene therapy 
650 4 |a rAAV 
650 4 |a tissue engineering 
650 7 |a Drug Carriers  |2 NLM 
650 7 |a Hydrogels  |2 NLM 
650 7 |a PEO-PPO-PEO  |2 NLM 
650 7 |a Propylene Glycols  |2 NLM 
650 7 |a Poloxamer  |2 NLM 
650 7 |a 106392-12-5  |2 NLM 
650 7 |a Polyethylene Glycols  |2 NLM 
650 7 |a 3WJQ0SDW1A  |2 NLM 
700 1 |a Gao, Liang  |e verfasserin  |4 aut 
700 1 |a Rey-Rico, Ana  |e verfasserin  |4 aut 
700 1 |a Venkatesan, Jagadeesh K  |e verfasserin  |4 aut 
700 1 |a Müller-Brandt, Kathrin  |e verfasserin  |4 aut 
700 1 |a Cai, Xiaoyu  |e verfasserin  |4 aut 
700 1 |a Goebel, Lars  |e verfasserin  |4 aut 
700 1 |a Schmitt, Gertrud  |e verfasserin  |4 aut 
700 1 |a Speicher-Mentges, Susanne  |e verfasserin  |4 aut 
700 1 |a Zurakowski, David  |e verfasserin  |4 aut 
700 1 |a Menger, Michael D  |e verfasserin  |4 aut 
700 1 |a Laschke, Matthias W  |e verfasserin  |4 aut 
700 1 |a Cucchiarini, Magali  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Advanced materials (Deerfield Beach, Fla.)  |d 1998  |g 32(2020), 2 vom: 20. Jan., Seite e1906508  |w (DE-627)NLM098206397  |x 1521-4095  |7 nnns 
773 1 8 |g volume:32  |g year:2020  |g number:2  |g day:20  |g month:01  |g pages:e1906508 
856 4 0 |u http://dx.doi.org/10.1002/adma.201906508  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |d 32  |j 2020  |e 2  |b 20  |c 01  |h e1906508