Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology

© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 71(2020), 4 vom: 19. Feb., Seite 1278-1293
1. Verfasser: Benninghaus, Vincent Alexander (VerfasserIn)
Weitere Verfasser: van Deenen, Nicole, Müller, Boje, Roelfs, Kai-Uwe, Lassowskat, Ines, Finkemeier, Iris, Prüfer, Dirk, Schulze Gronover, Christian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Taraxacum koksaghyz Articulated laticifers barnase/barstar differentially accumulated proteins latex-non-exuding phenotype metabolomics proteomics Latex Proteome
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520 |a Taraxacum koksaghyz has been identified as one of the most promising alternative rubber crops. Its high-quality rubber is produced in the latex of laticifers, a specialized cell type that is organized in a network of elongated tubules throughout the entire plant body. In order to gain insights into the physiological role(s) of latex and hence laticifer biology, we examine the effects of barnase-induced latex RNA degradation on the metabolite and protein compositions in the roots. We established high-quality datasets that enabled precise discrimination between cellular and physiological processes in laticifers and non-laticifer cell types of roots at different vegetative stages. We identified numerous latex-specific proteins, including a perilipin-like protein that has not been studied in plants yet. The barnase-expressing plants revealed a phenotype that did not exude latex, which may provide a valuable genetic basis for future studies of plant-environment interactions concerning latex and also help to clarify the evolution and arbitrary distribution of latex throughout the plant kingdom. The overview of temporal changes in composition and protein abundance provided by our data opens the way for a deeper understanding of the molecular interactions, reactions, and network relationships that underlie the different metabolic pathways in the roots of this potential rubber crop 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 4 |a Articulated laticifers 
650 4 |a barnase/barstar 
650 4 |a differentially accumulated proteins 
650 4 |a latex-non-exuding phenotype 
650 4 |a metabolomics 
650 4 |a proteomics 
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650 7 |a Proteome  |2 NLM 
700 1 |a van Deenen, Nicole  |e verfasserin  |4 aut 
700 1 |a Müller, Boje  |e verfasserin  |4 aut 
700 1 |a Roelfs, Kai-Uwe  |e verfasserin  |4 aut 
700 1 |a Lassowskat, Ines  |e verfasserin  |4 aut 
700 1 |a Finkemeier, Iris  |e verfasserin  |4 aut 
700 1 |a Prüfer, Dirk  |e verfasserin  |4 aut 
700 1 |a Schulze Gronover, Christian  |e verfasserin  |4 aut 
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