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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.9b01254
|2 doi
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|a pubmed24n1010.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Yates, Nicholas D
|e verfasserin
|4 aut
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| 245 |
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|a Aldehyde-Mediated Protein-to-Surface Tethering via Controlled Diazonium Electrode Functionalization Using Protected Hydroxylamines
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 26.08.2020
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|a Date Revised 26.08.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a We report a diazonium electro-grafting method for the covalent modification of conducting surfaces with aldehyde-reactive hydroxylamine functionalities that facilitate the wiring of redox-active (bio)molecules to electrode surfaces. Hydroxylamine near-monolayer formation is achieved via a phthalimide-protection and hydrazine-deprotection strategy that overcomes the multilayer formation that typically complicates diazonium surface modification. This surface modification strategy is characterized using electrochemistry (electrochemical impedance spectroscopy and cyclic voltammetry), X-ray photoelectron spectroscopy, and quartz crystal microbalance with dissipation monitoring. Thus-modified glassy carbon, boron-doped diamond, and gold surfaces are all shown to ligate to small molecule aldehydes, yielding surface coverages of 150-170, 40, and 100 pmol cm-2, respectively. Bioconjugation is demonstrated via the coupling of a dilute (50 μM) solution of periodate-oxidized horseradish peroxidase enzyme to a functionalized gold surface under biocompatible conditions (H2O solvent, pH 4.5, 25 °C)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Dowsett, Mark R
|e verfasserin
|4 aut
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|a Bentley, Phillip
|e verfasserin
|4 aut
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|a Dickenson-Fogg, Jack A
|e verfasserin
|4 aut
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|a Pratt, Andrew
|e verfasserin
|4 aut
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|a Blanford, Christopher F
|e verfasserin
|4 aut
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|a Fascione, Martin A
|e verfasserin
|4 aut
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|a Parkin, Alison
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 36(2020), 20 vom: 26. Mai, Seite 5654-5664
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:36
|g year:2020
|g number:20
|g day:26
|g month:05
|g pages:5654-5664
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|u http://dx.doi.org/10.1021/acs.langmuir.9b01254
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