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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.9b02414
|2 doi
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|a pubmed25n1008.xml
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|a (NLM)31663343
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ghosh, Anup Kumar
|e verfasserin
|4 aut
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|a Fabrication of Orange-Emitting Organic Nanoparticle-Protamine Conjugate
|b Fluorimetric Sensor of Heparin
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.07.2020
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|a Date Revised 22.07.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Among the diverse sensing techniques, fluorimetric detection dominates over the other methods because of its rapid signaling, high selectivity and sensitivity, and operational simplicity. This present article delineates fabrication of a fluorescent organic nanoparticle-protamine (FONP-Pro) conjugate for selective and sensitive detection of heparin simply by exploitation of the aggregation-induced emission (AIE) property of the FONPs. Naphthalene diimide-based bola-type amphiphilic molecules (NDI-1) comprise a naphthyl residue and a 3-aminopyridyl unit at both terminals, forming organic nanoparticles in a dimethyl sulfoxide-water binary solvent mixture, and exhibited AIE through excimer formation. The presence of naphthyl residue in the molecular backbone facilitates the intramolecular charge transfer to generate orange-emitting (λem = 594 nm) AIE-luminogen (AIE-gen). The aminopyridine residues within NDI-1 induced negative surface charge on NDI-1 FONPs, which facilitated interaction with positively charged protamine (Pro) to construct FONP-Pro conjugates. Formation of this NDI-1 FONP-Pro conjugate through the interaction between Pro and FONP drastically reduced the orange emission intensity (fluorescence off) of the AIE-gens. Interestingly, addition of heparin to this FONP-Pro conjugate turned on the fluorescence signal of FONPs through unwinding of the Pro from the FONP surface because of a strong binding affinity between heparin and Pro. Formation of the FONP-Pro conjugate and fluorimetric sensing of heparin was investigated by monitoring the change in emission behavior of NDI-1 FONPs. Also, the heparin-sensing was found to be highly selective against many other biomolecules including proteins, enzymes, and DNA. Hence, a selective and efficient heparin sensor (FONP-Pro) was developed having a limit of detection of 12 nM simply by utilizing the fluorescence "turn-off" and "turn-on" mechanism of NDI-1 FONP
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fluorescent Dyes
|2 NLM
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|a Naphthalimides
|2 NLM
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|a Protamines
|2 NLM
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|a Heparin
|2 NLM
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|a 9005-49-6
|2 NLM
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|a Choudhury, Pritam
|e verfasserin
|4 aut
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|a Das, Prasanta Kumar
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 35(2019), 47 vom: 26. Nov., Seite 15180-15191
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:35
|g year:2019
|g number:47
|g day:26
|g month:11
|g pages:15180-15191
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|u http://dx.doi.org/10.1021/acs.langmuir.9b02414
|3 Volltext
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