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|a eng
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| 100 |
1 |
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|a Thiombane, Ndeye Khady
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Single-cell analysis for drug development using convex lens-induced confinement imaging
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|c 2019
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|a Text
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|a ƒaComputermedien
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|a Date Completed 07.07.2020
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|a Date Revised 07.07.2020
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|a published: Print-Electronic
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|a ErratumIn: Biotechniques. 2020 Jan;68(1):52. doi: 10.2144/btn-2019-0067e1. - PMID 31813232
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|a Citation Status MEDLINE
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|a New technologies have powered rapid advances in cellular imaging, genomics and phenotypic analysis in life sciences. However, most of these methods operate at sample population levels and provide statistical averages of aggregated data that fail to capture single-cell heterogeneity, complicating drug discovery and development. Here we demonstrate a new single-cell approach based on convex lens-induced confinement (CLiC) microscopy. We validated CLiC on yeast cells, demonstrating subcellular localization with an enhanced signal-to-noise and fluorescent signal detection sensitivity compared with traditional imaging. In the live-cell CLiC assay, cellular proliferation times were consistent with flask culture. Using methotrexate, we provide drug response data showing a fivefold cell size increase following drug exposure. Taken together, CLiC enables high-quality imaging of single-cell drug response and proliferation for extended observation periods
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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4 |
|a CLiC
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| 650 |
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4 |
|a dose-response
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| 650 |
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4 |
|a methotrexate
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| 650 |
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4 |
|a phenotypic screen
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| 650 |
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4 |
|a single cell
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| 650 |
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4 |
|a yeast
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| 700 |
1 |
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|a Coutin, Nicolas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Berard, Daniel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tahvildari, Radin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Leslie, Sabrina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nislow, Corey
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 67(2019), 5 vom: 01. Nov., Seite 210-217
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:67
|g year:2019
|g number:5
|g day:01
|g month:11
|g pages:210-217
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| 856 |
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|u http://dx.doi.org/10.2144/btn-2019-0067
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