Abnormal Sysmex XT-2000iV DIFF scattergram in a cat with a prominent mastocytemia

© 2019 American Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 48(2019), 4 vom: 24. Dez., Seite 624-629
1. Verfasser: Lavabre, Typhaine (VerfasserIn)
Weitere Verfasser: Betting, Adeline, Bourgès-Abella, Nathalie, Layssol-Lamour, Catherine, Trumel, Catherine
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Case Reports Journal Article Differential leukocyte count feline blood laser counter lysis-resistant granulocytes mastocytosis
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520 |a A 2-year-old neutered male domestic shorthair cat was presented to the emergency service of the National Veterinary School of Toulouse (France) for acute vomiting and diarrhea with lethargy, inappetence, and adypsia for the past 48 hours. Complete blood counts were performed with the ProCyte DX at the emergency department and with the Sysmex XT-2000iV at the laboratory 2 weeks later. The scattergrams from the two analyzers revealed similar unusual and abnormal dot plots. The Sysmex XT-2000iV DIFF scattergram also showed no clear separation between different leukocyte populations. The eosinophil cluster was in an abnormal location compared with that of the "typical" location in a normal cat. A blood smear evaluation revealed the presence of numerous mast cells. Thus, we hypothesized that the Sysmex XT-2000iV had detected the mast cell population, and this led to errors in the differential counts. To explore this hypothesis, we manually gated on the DIFF scattergram and performed a manual differential on the blood smear. With this new gating strategy, the Sysmex XT-2000iV and manual differentials were similar. Thus, in the case of systemic mastocytosis, mast cells can be located between the lymphocyte, monocyte, and eosinophil clusters on scattergrams 
650 4 |a Case Reports 
650 4 |a Journal Article 
650 4 |a Differential leukocyte count 
650 4 |a feline blood 
650 4 |a laser counter 
650 4 |a lysis-resistant granulocytes 
650 4 |a mastocytosis 
700 1 |a Betting, Adeline  |e verfasserin  |4 aut 
700 1 |a Bourgès-Abella, Nathalie  |e verfasserin  |4 aut 
700 1 |a Layssol-Lamour, Catherine  |e verfasserin  |4 aut 
700 1 |a Trumel, Catherine  |e verfasserin  |4 aut 
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