Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants

Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 225(2020), 5 vom: 23. März, Seite 2166-2182
1. Verfasser: Munakata, Ryosuke (VerfasserIn)
Weitere Verfasser: Kitajima, Sakihito, Nuttens, Andréïna, Tatsumi, Kanade, Takemura, Tomoya, Ichino, Takuji, Galati, Gianni, Vautrin, Sonia, Bergès, Hélène, Grosjean, Jérémy, Bourgaud, Frédéric, Sugiyama, Akifumi, Hehn, Alain, Yazaki, Kazufumi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Moraceae UbiA superfamily convergent evolution fig (Ficus carica) furanocoumarin latex prenyltransferase Furocoumarins mehr... Latex Dimethylallyltranstransferase EC 2.5.1.1
Beschreibung
Zusammenfassung:© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
Furanocoumarins (FCs) are plant-specialized metabolites with potent allelochemical properties. The distribution of FCs is scattered with a chemotaxonomical tendency towards four distant families with highly similar FC pathways. The mechanism by which this pathway emerged and spread in plants has not been elucidated. Furanocoumarin biosynthesis was investigated in Ficus carica (fig, Moraceae), focusing on the first committed reaction catalysed by an umbelliferone dimethylallyltransferase (UDT). Comparative RNA-seq analysis among latexes of different fig organs led to the identification of a UDT. The phylogenetic relationship of this UDT to previously reported Apiaceae UDTs was evaluated. The expression pattern of F. carica prenyltransferase 1 (FcPT1) was related to the FC contents in different latexes. Enzymatic characterization demonstrated that one of the main functions of FcPT1 is UDT activity. Phylogenetic analysis suggested that FcPT1 and Apiaceae UDTs are derived from distinct ancestors, although they both belong to the UbiA superfamily. These findings are supported by significant differences in the related gene structures. This report describes the identification of FcPT1 involved in FC biosynthesis in fig and provides new insights into multiple origins of the FC pathway and, more broadly, into the adaptation of plants to their environments
Beschreibung:Date Completed 14.05.2021
Date Revised 14.05.2021
published: Print-Electronic
GENBANK: BDEM01000717.1, BDEM01000270.1, BDEM01000105.1
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16277