Comparative phosphoproteomic analysis of developing maize seeds suggests a pivotal role for enolase in promoting starch synthesis

Copyright © 2019. Published by Elsevier B.V.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 289(2019) vom: 22. Dez., Seite 110243
1. Verfasser: Cao, Hanwei (VerfasserIn)
Weitere Verfasser: Zhou, Yuwei, Chang, Ying, Zhang, Xiuyan, Li, Cui, Ren, Dongtao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Comparative Study Journal Article Enolase Maize seeds Phosphoproteomics Starch synthesis Phosphoproteins Plant Proteins Proteome Starch mehr... 9005-25-8 Phosphopyruvate Hydratase EC 4.2.1.11
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520 |a Maize (Zea mays) seeds are the major source of starch all over the world and the excellent model for researching starch synthesis. Seed starch content is a typical quantitative phenotype and many reports revealed that the glycolytic enzymes are involved in regulating starch synthesis, however the regulatory mechanism is still unclear. Here, we present a comparative phosphoproteomic study of three maize inbred lines with different seed starch content. It reveals that abundances of 62 proteins and 63 phosphoproteins were regulated during maize seed development. Dynamics of 17 enzymes related to glycolysis and starch synthesis were used to construct a phosphorylation regulatory network of starch synthesis. It shows that starch synthesis and glycolysis in maize seeds utilize the same hexose phosphates pool coming from sorbitol and sucrose as carbon source, and phosphorylation of ZmENO1 are suggested to contribute to increase starch content, because it is positively related to seed starch content in different developmental stages and different lines, and the phosphor-mimic mutant (ZmENO1S43D) damaged its enzyme activity which is vital in glycolysis. Our results provide a new sight into regulatory process of seed starch synthesis and can be used in maize breeding for high starch content 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Enolase 
650 4 |a Maize seeds 
650 4 |a Phosphoproteomics 
650 4 |a Starch synthesis 
650 7 |a Phosphoproteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Proteome  |2 NLM 
650 7 |a Starch  |2 NLM 
650 7 |a 9005-25-8  |2 NLM 
650 7 |a Phosphopyruvate Hydratase  |2 NLM 
650 7 |a EC 4.2.1.11  |2 NLM 
700 1 |a Zhou, Yuwei  |e verfasserin  |4 aut 
700 1 |a Chang, Ying  |e verfasserin  |4 aut 
700 1 |a Zhang, Xiuyan  |e verfasserin  |4 aut 
700 1 |a Li, Cui  |e verfasserin  |4 aut 
700 1 |a Ren, Dongtao  |e verfasserin  |4 aut 
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773 1 8 |g volume:289  |g year:2019  |g day:22  |g month:12  |g pages:110243 
856 4 0 |u http://dx.doi.org/10.1016/j.plantsci.2019.110243  |3 Volltext 
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