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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2019.110243
|2 doi
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|a pubmed25n1007.xml
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|a (DE-627)NLM302301488
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|a (NLM)31623796
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|a (PII)S0168-9452(19)30849-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Cao, Hanwei
|e verfasserin
|4 aut
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|a Comparative phosphoproteomic analysis of developing maize seeds suggests a pivotal role for enolase in promoting starch synthesis
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.01.2020
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019. Published by Elsevier B.V.
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|a Maize (Zea mays) seeds are the major source of starch all over the world and the excellent model for researching starch synthesis. Seed starch content is a typical quantitative phenotype and many reports revealed that the glycolytic enzymes are involved in regulating starch synthesis, however the regulatory mechanism is still unclear. Here, we present a comparative phosphoproteomic study of three maize inbred lines with different seed starch content. It reveals that abundances of 62 proteins and 63 phosphoproteins were regulated during maize seed development. Dynamics of 17 enzymes related to glycolysis and starch synthesis were used to construct a phosphorylation regulatory network of starch synthesis. It shows that starch synthesis and glycolysis in maize seeds utilize the same hexose phosphates pool coming from sorbitol and sucrose as carbon source, and phosphorylation of ZmENO1 are suggested to contribute to increase starch content, because it is positively related to seed starch content in different developmental stages and different lines, and the phosphor-mimic mutant (ZmENO1S43D) damaged its enzyme activity which is vital in glycolysis. Our results provide a new sight into regulatory process of seed starch synthesis and can be used in maize breeding for high starch content
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|a Comparative Study
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|a Journal Article
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|a Enolase
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|a Maize seeds
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|a Phosphoproteomics
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|a Starch synthesis
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|a Phosphoproteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Proteome
|2 NLM
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|a Starch
|2 NLM
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|a 9005-25-8
|2 NLM
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|a Phosphopyruvate Hydratase
|2 NLM
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|a EC 4.2.1.11
|2 NLM
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|a Zhou, Yuwei
|e verfasserin
|4 aut
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1 |
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|a Chang, Ying
|e verfasserin
|4 aut
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|a Zhang, Xiuyan
|e verfasserin
|4 aut
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|a Li, Cui
|e verfasserin
|4 aut
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|a Ren, Dongtao
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 289(2019) vom: 22. Dez., Seite 110243
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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|g volume:289
|g year:2019
|g day:22
|g month:12
|g pages:110243
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|u http://dx.doi.org/10.1016/j.plantsci.2019.110243
|3 Volltext
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|d 289
|j 2019
|b 22
|c 12
|h 110243
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