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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2019.09.044
|2 doi
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|a pubmed24n1224.xml
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|a (NLM)31622940
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|a (PII)S0981-9428(19)30396-1
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Kumari, Khushboo
|e verfasserin
|4 aut
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|a Analysis of γ-Tocopherol methyl transferase3 promoter activity and study of methylation patterns of the promoter and its gene body
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.02.2020
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
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|a Soybeans are known for its good source of protein (40%), oil (20%) and also serve as a source of nutraceutical compounds including tocopherols (toc). To know the molecular basis of differential α-toc accumulation in two contrasting soybean genotypes: DS74 (low α-toc - 1.36 μg/g and total-toc -29.72 μg/g) and Bragg (high α-toc - 10.48 μg/g and total-toc 178.91 μg/g), the analysis of γ-TMT3 promoter activity and its methylation patterns were carried out. The sequencing results revealed nucleotide variation between Bragg:γ-TMT3-P and DS74:γ-TMT3-P, however none of the variations were found in core-promoter region or in cis-elements. The histochemical GUS assay revealed higher promoter activity of Bragg:γ-TMT3-P than that of DS74:γ-TMT3-P and correlated with significantly higher and lower (P < 0.05) expression of γ-TMT3 gene respectively. To know the molecular basis of differential accumulation of α-toc in these contrasting soybean genotypes, the DNA methylation pattern of γ-TMT3 gene body and its promoter was studied in both varieties. The results showed higher percentage (62.5%) of methylation in DS74:γ-TMT3-P than in Bragg:γ-TMT3-P (50%). Out of all the methylation sites in the promoter region, one of methylation site was found at CAAT box (-190 bp) of DS74:γ-TMT3-P. Further gene body methylation patterns revealed lowest % (40%) of CG methylation in DS74:γ-TMT3 gene as compared to Bragg:γ-TMT3 (64.2%). Thus our study revealed that, expression of γ-TMT3 gene was influenced by its promoter activity and methylation patterns in cis-elements of γ-TMT3 promoter and gene body. This study will help us to understand the possible role of methylation and promoter activity in determining the α-toc content in soybean seeds
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|a Journal Article
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|a Gene body methylation
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|a Gene expression
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|a Methylation
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|a Promoter
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|a Tocopherol biosynthesis
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|a cis-regulatory elements
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|a α-tocopherol
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|a gamma-Tocopherol
|2 NLM
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|a 8EF1Z1238F
|2 NLM
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| 650 |
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|a alpha-Tocopherol
|2 NLM
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| 650 |
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|a H4N855PNZ1
|2 NLM
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|a Tocopherols
|2 NLM
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|a R0ZB2556P8
|2 NLM
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|a Rai, Monika Prakash
|e verfasserin
|4 aut
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|a Bansal, Navita
|e verfasserin
|4 aut
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|a Rama Prashat, G
|e verfasserin
|4 aut
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|a Kumari, Sweta
|e verfasserin
|4 aut
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|a Krishnan, Veda
|e verfasserin
|4 aut
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|a Srivathsa, Rohini
|e verfasserin
|4 aut
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|a Dahuja, Anil
|e verfasserin
|4 aut
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|a Sachdev, Archana
|e verfasserin
|4 aut
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|a Praveen, Shelly
|e verfasserin
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|a Vinutha, T
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 144(2019) vom: 17. Nov., Seite 375-385
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:144
|g year:2019
|g day:17
|g month:11
|g pages:375-385
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|u http://dx.doi.org/10.1016/j.plaphy.2019.09.044
|3 Volltext
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|d 144
|j 2019
|b 17
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|h 375-385
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