Overexpression of heterotrimeric G protein beta subunit gene (OsRGB1) confers both heat and salinity stress tolerance in rice
Copyright © 2019 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 144(2019) vom: 01. Nov., Seite 334-344 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2019
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Abiotic stress Gene expression Heat and salinity tolerance Heterotrimeric G protein beta In planta transformation Stress responsive genes Plant Proteins |
| Zusammenfassung: | Copyright © 2019 Elsevier Masson SAS. All rights reserved. Constitutive overexpression of the rice heterotrimeric G protein beta subunit gene (RGB1) in the commercial rice cultivar BRRI Dhan 55 resulted in improved tolerance to heat or salinity or their combination. Two independently in planta transformed plants with the gene confirmed to be integrated at T2 by Southern hybridization and showing high expression at the T3 seedling stage showed better physiological performance after 8 days in 120 mM salt stress than the wild type. The plants had significantly lower electrolyte leakage and malondialdehyde production, while showing higher levels of chlorophyll. Significantly higher germination at 48 °C or with combined stresses of 42/40 °C day/night stress in the presence of 120 mM salt for 2 days was also observed. Stress responsive genes such as OsAPX1, OsSOD, OsHKT1, OsHSP1, OsHSP2 and OsCOR47 showed higher expression in the RGB1 positive plants. These RGB1 transgenic plants can likely provide a strong defense against climate change |
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| Beschreibung: | Date Completed 20.02.2020 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2019.10.005 |