The Myb-like transcription factor phosphorus starvation response (PtPSR) controls conditional P acquisition and remodelling in marine microalgae

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 225(2020), 6 vom: 15. März, Seite 2380-2395
1. Verfasser:	Kumar Sharma, Amit (VerfasserIn)
Weitere Verfasser:	Mühlroth, Alice, Jouhet, Juliette, Maréchal, Eric, Alipanah, Leila, Kissen, Ralph, Brembu, Tore, Bones, Atle M, Winge, Per
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Myb transcription factor alkaline phosphatase (AP) diatom lipid remodelling phosphate stress response phosphorus reshuffling of phosphate containing compounds Transcription Factors mehr... Phosphorus 27YLU75U4W

Beschreibung
Zusammenfassung:	© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. Phosphorus (P) is one of the limiting macronutrients for algal growth in marine environments. Microalgae have developed adaptation mechanisms to P limitation that involve remodelling of internal phosphate resources and accumulation of lipids. Here, we used in silico analyses to identify the P-stress regulator PtPSR (Phaeodactylum tricornutum phosphorus starvation response) in the diatom P. tricornutum. ptpsr mutant lines were generated using gene editing and characterised by various molecular, genetics and biochemical tools. PtPSR belongs to a clade of Myb transcription factors that are conserved in stramenopiles and distantly related to plant P-stress regulators. PtPSR bound specifically to a conserved cis-regulatory element found in the regulatory region of P-stress-induced genes. ptpsr knockout mutants showed reduction in cell growth under P limitation. P-stress responses were impaired in ptpsr mutants compared with wild-type, including reduced induction of P-stress response genes, near to complete loss of alkaline phosphatase activity and reduced phospholipid degradation. We conclude that PtPSR is a key transcription factor influencing P scavenging, phospholipid remodelling and cell growth in adaptation to P stress in diatoms
Beschreibung:	Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.16248