Differential phosphorylation of the N-terminal extension regulates phytochrome B signaling

Differential phosphorylation of the N-terminal extension regulates phytochrome B signaling

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 225(2020), 4 vom: 09. Feb., Seite 1635-1650
1. Verfasser: Viczián, András (VerfasserIn)
Weitere Verfasser: Ádám, Éva, Staudt, Anne-Marie, Lambert, Dorothee, Klement, Eva, Romero Montepaone, Sofia, Hiltbrunner, Andreas, Casal, Jorge, Schäfer, Eberhard, Nagy, Ferenc, Klose, Cornelia
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't dark reversion phosphorylation phyB NTE phytochrome thermal reversion Apoproteins Arabidopsis Proteins PHYE protein, Arabidopsis mehr... Phytochrome 11121-56-5 Phytochrome B 136250-22-1 PHYD protein, Arabidopsis 158379-16-9
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520 |a Phytochrome B (phyB) is an excellent light quality and quantity sensor that can detect subtle changes in the light environment. The relative amounts of the biologically active photoreceptor (phyB Pfr) are determined by the light conditions and light independent thermal relaxation of Pfr into the inactive phyB Pr, termed thermal reversion. Little is known about the regulation of thermal reversion and how it affects plants' light sensitivity. In this study we identified several serine/threonine residues on the N-terminal extension (NTE) of Arabidopsis thaliana phyB that are differentially phosphorylated in response to light and temperature, and examined transgenic plants expressing nonphosphorylatable and phosphomimic phyB mutants. The NTE of phyB is essential for thermal stability of the Pfr form, and phosphorylation of S86 particularly enhances the thermal reversion rate of the phyB Pfr-Pr heterodimer in vivo. We demonstrate that S86 phosphorylation is especially critical for phyB signaling compared with phosphorylation of the more N-terminal residues. Interestingly, S86 phosphorylation is reduced in light, paralleled by a progressive Pfr stabilization under prolonged irradiation. By investigating other phytochromes (phyD and phyE) we provide evidence that acceleration of thermal reversion by phosphorylation represents a general mechanism for attenuating phytochrome signaling 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a dark reversion 
650 4 |a phosphorylation 
650 4 |a phyB NTE 
650 4 |a phytochrome 
650 4 |a thermal reversion 
650 7 |a Apoproteins  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a PHYE protein, Arabidopsis  |2 NLM 
650 7 |a Phytochrome  |2 NLM 
650 7 |a 11121-56-5  |2 NLM 
650 7 |a Phytochrome B  |2 NLM 
650 7 |a 136250-22-1  |2 NLM 
650 7 |a PHYD protein, Arabidopsis  |2 NLM 
650 7 |a 158379-16-9  |2 NLM 
700 1 |a Ádám, Éva  |e verfasserin  |4 aut 
700 1 |a Staudt, Anne-Marie  |e verfasserin  |4 aut 
700 1 |a Lambert, Dorothee  |e verfasserin  |4 aut 
700 1 |a Klement, Eva  |e verfasserin  |4 aut 
700 1 |a Romero Montepaone, Sofia  |e verfasserin  |4 aut 
700 1 |a Hiltbrunner, Andreas  |e verfasserin  |4 aut 
700 1 |a Casal, Jorge  |e verfasserin  |4 aut 
700 1 |a Schäfer, Eberhard  |e verfasserin  |4 aut 
700 1 |a Nagy, Ferenc  |e verfasserin  |4 aut 
700 1 |a Klose, Cornelia  |e verfasserin  |4 aut 
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773 1 8 |g volume:225  |g year:2020  |g number:4  |g day:09  |g month:02  |g pages:1635-1650 
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