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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1080/02786826.2018.1560391
|2 doi
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|a pubmed24n1006.xml
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|a (NLM)31588161
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Berg, Kathleen E
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Electrochemical Dithiothreitol Assay for Large-Scale Particulate Matter Studies
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 25.01.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Particulate matter (PM) air pollution is associated with human morbidity and mortality. Measuring PM oxidative potential has been shown to provide a predictive measurement between PM exposure and adverse health impacts. The dithiothreitol (DTT) assay is commonly used to measure the oxidative potential of PM2.5 (PM less than 2.5 μm aerodynamic diameter). In the common, kinetic form of this assay, the decay of DTT is quantified over time (indirectly) using 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman's reagent) via UV/vis absorbance spectroscopy. The loss of DTT can also be quantified directly using electrochemical detection. The objectives of this work were (1) to evaluate the electrochemical assay, using commercially available equipment, relative to the UV/vis absorbance assay, and (2) to apply the electrochemical method to a large (>100) number of PM2.5 aerosol filter samples. Also presented here is the comparison an end-point assay to the kinetic assay, in an attempt to reduce the time, labor, and materials neccssary to quantify PM oxidative potential. The end-point, electrochemical assay gave comparable results to the UV/vis absorbance assay for PM filter sample analysis. Finally, high filter mass loadings (higher than about 0.5 μg PM per mm2 filter) lead to sub-optimal DTT assay performance, which suggests future studies should limit particle mass loadings on filters
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|a Journal Article
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1 |
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|a Turner, Laurelle R
|e verfasserin
|4 aut
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1 |
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|a Benka-Coker, Megan L
|e verfasserin
|4 aut
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1 |
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|a Rajkumar, Sarah
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Young, Bonnie N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Peel, Jennifer L
|e verfasserin
|4 aut
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1 |
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|a Clark, Maggie L
|e verfasserin
|4 aut
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1 |
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|a Volckens, John
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Henry, Charles S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Aerosol science and technology : the journal of the American Association for Aerosol Research
|d 1989
|g 53(2019), 3 vom: 14., Seite 268-275
|w (DE-627)NLM09822025X
|x 0278-6826
|7 nnns
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| 773 |
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|g volume:53
|g year:2019
|g number:3
|g day:14
|g pages:268-275
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|u http://dx.doi.org/10.1080/02786826.2018.1560391
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