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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erz445
|2 doi
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|a pubmed24n1006.xml
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|a (DE-627)NLM301942366
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|a (NLM)31587070
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Niu, Wei-Tao
|e verfasserin
|4 aut
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| 245 |
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|a Arabidopsis cyclic nucleotide-gated channel 6 is negatively modulated by multiple calmodulin isoforms during heat shock
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.03.2021
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|a Date Revised 05.03.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a An increased concentration of cytosolic Ca2+ is an early response of plant cells to heat shock. Arabidopsis cyclic nucleotide-gated ion channel 6 (CNGC6) mediates heat-induced Ca2+ influx and is activated by cAMP. However, it remains unclear how the Ca2+ conductivity of CNGC6 is negatively regulated under the elevated cytosolic Ca2+ concentration. In this study, Arabidopsis calmodulin isoforms CaM1/4, CaM2/3/5, CaM6, and CaM7 were found to bind to CNGC6 to varying degrees, and this binding was dependent on the presence of Ca2+ and IQ6, an atypical isoleucine-glutamine motif in CNGC6. Knockout of CaM2, CaM3, CaM5, and CaM7 genes led to a marked increase in plasma membrane inward Ca2+ current under heat shock conditions; however, knockout of CaM1, CaM4, and CaM6 genes had no significant effect on plasma membrane Ca2+ current. Moreover, the deletion of IQ6 from CNGC6 led to a marked increase in plasma membrane Ca2+ current under heat shock conditions. Taken together, the data suggest that CNGC6-mediated Ca2+ influx is likely to be negatively regulated by CaM2/3/5 and CaM7 isoforms under heat shock conditions, and that IQ6 plays an important role in CaM binding and the feedback regulation of the channel
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis, Ca2+ conductance
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|a CNGC6
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|a CaM
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|a CaM binding domain
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|a heat shock
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a CNGC6 protein, Arabidopsis
|2 NLM
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| 650 |
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|a Calmodulin
|2 NLM
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| 650 |
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|a Cyclic Nucleotide-Gated Cation Channels
|2 NLM
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| 650 |
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|a Protein Isoforms
|2 NLM
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| 700 |
1 |
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|a Han, Xiao-Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wei, Shan-Shan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Shang, Zhong-Lin
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, De-Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fan, Xiao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gao, Fei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Shu-Zhi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bai, Jiao-Teng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Zi-Xuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Bing
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 71(2020), 1 vom: 01. Jan., Seite 90-104
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:71
|g year:2020
|g number:1
|g day:01
|g month:01
|g pages:90-104
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|u http://dx.doi.org/10.1093/jxb/erz445
|3 Volltext
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|d 71
|j 2020
|e 1
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|h 90-104
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