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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2019.09.045
|2 doi
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|a pubmed24n1006.xml
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|a (DE-627)NLM301925763
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|a (NLM)31585398
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|a (PII)S0981-9428(19)30397-3
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Parrotta, Luigi
|e verfasserin
|4 aut
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|a A low molecular-weight cyclophilin localizes in different cell compartments of Pyrus communis pollen and is released in vitro under Ca2+ depletion
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.02.2020
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
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|a Cyclophilins (CyPs) are ubiquitous proteins involved in a wide variety of processes including protein maturation and trafficking, receptor complex stabilization, apoptosis, receptor signaling, RNA processing, and spliceosome assembly. The ubiquitous presence is justified by their peptidyl-prolyl cis-trans isomerase (PPIase) activity, catalyzing the rotation of X-Pro peptide bonds from a cis to a trans conformation, a critical rate-limiting step in protein folding, as over 90% of proteins contain trans prolyl imide bonds. In Arabidopsis 35 CyPs involved in plant development have been reported, showing different subcellular localizations and tissue- and stage-specific expression. In the present work, we focused on the localization of CyPs in pear (Pyrus communis) pollen, a model system for studies on pollen tube elongation and on pollen-pistil self-incompatibility response. Fluorescent, confocal and immuno-electron microscopy showed that this protein is present in the cytoplasm, organelles and cell wall, as confirmed by protein fractionation. Moreover, an 18-kDa CyP isoform was specifically released extracellularly when pear pollen was incubated with the Ca2+ chelator EGTA
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|a Journal Article
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|a Cyclophilin
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|a EGTA
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|a Pollen tube
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|a Pyrus communis
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|a Cyclophilins
|2 NLM
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|a EC 5.2.1.-
|2 NLM
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|a Calcium
|2 NLM
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|a SY7Q814VUP
|2 NLM
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| 700 |
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|a Aloisi, Iris
|e verfasserin
|4 aut
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|a Suanno, Chiara
|e verfasserin
|4 aut
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|a Faleri, Claudia
|e verfasserin
|4 aut
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|a Kiełbowicz-Matuk, Agnieszka
|e verfasserin
|4 aut
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|a Bini, Luca
|e verfasserin
|4 aut
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|a Cai, Giampiero
|e verfasserin
|4 aut
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|a Del Duca, Stefano
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 144(2019) vom: 15. Nov., Seite 197-206
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:144
|g year:2019
|g day:15
|g month:11
|g pages:197-206
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|u http://dx.doi.org/10.1016/j.plaphy.2019.09.045
|3 Volltext
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|d 144
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