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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1155/2019/1247276
|2 doi
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|a pubmed24n1006.xml
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wei, Lei
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Wnt1 Promotes EAAT2 Expression and Mediates the Protective Effects of Astrocytes on Dopaminergic Cells in Parkinson's Disease
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.07.2020
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|a Date Revised 27.07.2020
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|a published: Electronic-eCollection
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|a Citation Status MEDLINE
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|a Copyright © 2019 Lei Wei et al.
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|a Background: Wnt/β-catenin signaling has been reported to exert cytoprotective effects in a cellular model of Parkinson's disease (PD). Glutamate excitotoxicity has been suggested to contribute to the pathogenesis of PD, and excitatory amino acid transporters (EAATs) play a predominant role in clearing excessive glutamate. EAAT2 is mainly expressed in astrocytes, which are an important source of Wnt signaling in the brain
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|a Methods: Wnt1-overexpressing U251 astrocytes were indirectly cocultured with dopaminergic SH-SY5Y cells treated with 6-hydroxydopamine (6-OHDA). Cell toxicity was determined by cell viability and flow cytometric detection. Glutamate level in the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). Western blot analysis was used to detect the expression of Wnt1, β-catenin, and EAAT2. Immunofluorescence was used to display the expression and translocation of NF-κB p65
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|a Results: 6-OHDA treatment significantly decreased cell viability in both U251 cells and SH-SY5Y cells, inhibited the expression of Wnt1, β-catenin, and EAAT2 in U251 cells, and increased the glutamate level in the culture medium. Coculture with Wnt1-overexpressing U251 cells attenuated 6-OHDA-induced apoptosis in SH-SY5Y cells. Overexpression of Wnt1 decreased the glutamate level in the culture media, upregulated β-catenin, EAAT2, and NF-κB levels, and promoted the translocation of NF-κB from the cytoplasm to the nucleus in U251 cells
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|a Conclusion: Wnt1 promoted EAAT2 expression and mediated the cytoprotective effects of astrocytes on dopaminergic cells. NF-κB might be involved in the regulation of EAAT2 by Wnt1
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a CTNNB1 protein, human
|2 NLM
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|a Excitatory Amino Acid Transporter 2
|2 NLM
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| 650 |
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|a Wnt1 Protein
|2 NLM
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| 650 |
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|a beta Catenin
|2 NLM
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| 650 |
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|a Glutamic Acid
|2 NLM
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| 650 |
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|a 3KX376GY7L
|2 NLM
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|a Oxidopamine
|2 NLM
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| 650 |
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|a 8HW4YBZ748
|2 NLM
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| 700 |
1 |
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|a Chen, Chuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ding, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mo, Mingshu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zou, Jing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Zhenze
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Haiyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Haotian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dai, Yongqiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Pingyi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Zhengqi
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Neural plasticity
|d 1998
|g 2019(2019) vom: 24., Seite 1247276
|w (DE-627)NLM098558390
|x 1687-5443
|7 nnns
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| 773 |
1 |
8 |
|g volume:2019
|g year:2019
|g day:24
|g pages:1247276
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| 856 |
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|u http://dx.doi.org/10.1155/2019/1247276
|3 Volltext
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