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231225s2020 xx |||||o 00| ||eng c |
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|a 10.1111/nph.16233
|2 doi
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|a pubmed25n1005.xml
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|a (DE-627)NLM301815739
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|a (NLM)31574168
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wang, Wen-Qiu
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Genome-wide analysis of coding and non-coding RNA reveals a conserved miR164-NAC regulatory pathway for fruit ripening
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|c 2020
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 08.04.2021
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|a Date Revised 08.04.2021
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|a published: Print-Electronic
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|a GENBANK: PRJNA545413, SRR6885590, SRR6885601, EF063572, MK976922, 55206, PRJNA445209
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|a Citation Status MEDLINE
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| 520 |
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|a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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| 520 |
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|a Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene-responsive structural genes and transcription factors. However, the roles of other post-transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High-throughput sequencing sRNAome, degradome and transcriptome methods were used to identify further contributors to ripening control in the kiwifruit (A. deliciosa cv 'Hayward'). Two NAM/ATAF/CUC domain transcription factors (AdNAC6 and AdNAC7), both predicted targets for miR164, showed significant upregulation by exogenous ethylene. Gene expression analysis and luciferase reporter assays indicated that Ade-miR164 and one of its precursor miRNAs (Ade-MIR164b) were repressed by ethylene treatment and negatively correlated with AdNAC6/7 expression. Subsequent analysis indicated that both AdNAC6 and AdNAC7 proteins are transcriptional activators and physically bind the promoters of AdACS1 (1-aminocyclopropane-1-carboxylate synthase), AdACO1 (1-aminocyclopropane-1-carboxylic acid oxidase), AdMAN1 (endo-β-mannanase) and AaTPS1 (terpene synthase). Moreover, subcellular analysis indicated that the location of the AdNAC6/7 proteins was influenced by Ade-miR164. Multiple omics-based approaches revealed a novel regulatory link for fruit ripening that involved ethylene-miR164-NAC. The regulatory pathway for miR164-NAC is present in various fruit (e.g. Rosaceae fruit, citrus, grape), with implications for fruit ripening regulation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a NAC
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|a aroma
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|a cell wall
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|a degradome
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| 650 |
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|a ethylene
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| 650 |
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|a fruit ripening
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| 650 |
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|a miR164
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| 650 |
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|a transcriptome
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| 650 |
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|a DNA, Plant
|2 NLM
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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|a RNA, Plant
|2 NLM
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| 650 |
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|a RNA, Untranslated
|2 NLM
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| 700 |
1 |
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|a Wang, Jian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Ying-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Da-Wei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Allan, Andrew C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yin, Xue-Ren
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1984
|g 225(2020), 4 vom: 01. Feb., Seite 1618-1634
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
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|g volume:225
|g year:2020
|g number:4
|g day:01
|g month:02
|g pages:1618-1634
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.16233
|3 Volltext
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|d 225
|j 2020
|e 4
|b 01
|c 02
|h 1618-1634
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