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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-19-0773-RE
|2 doi
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|a pubmed24n1005.xml
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|a eng
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| 100 |
1 |
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|a Wang, Q W
|e verfasserin
|4 aut
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| 245 |
1 |
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|a q-LAMP Assays for the Detection of Botryosphaeria dothidea Causing Chinese Hickory Canker in Trunk, Water, and Air Samples
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| 264 |
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|c 2019
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| 336 |
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 27.11.2019
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|a Date Revised 27.11.2019
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Trunk canker disease caused by Botryosphaeria dothidea with a prolonged latent infection phase poses a serious threat to Chinese hickory production. To further understand the epidemiological characteristics and develop reasonable management techniques, a quantitative loop-mediated isothermal amplification (q-LAMP) assay was developed to quantitatively monitor B. dothidea in hickory plants, water, and air samples. Specific primers were designed based on the different sites of the β-tubulin sequence between B. dothidea and other fungi commonly found on Chinese hickory. At the optimum reaction temperature of 65.9°C, this loop-mediated isothermal amplification (LAMP) assay can specifically distinguish B. dothidea from other tested fungi. The limit of detection of LAMP assays for B. dothidea was 0.001 ng/µl of pure genomic DNA and 10 spores per 1 ml of water. The q-LAMP assay enables rapid detection of B. dothidea within 60 min in hickory trunk, water in hickory forests, and spores captured on tapes. These results provide a powerful and convenient tool for monitoring B. dothidea, which could be applied widely in epidemiology, forecast, and management of tree canker disease
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a pathogen detection
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| 650 |
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4 |
|a tree nuts
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| 700 |
1 |
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|a Zhang, C Q
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant disease
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|g 103(2019), 12 vom: 25. Dez., Seite 3142-3149
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|g volume:103
|g year:2019
|g number:12
|g day:25
|g month:12
|g pages:3142-3149
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|u http://dx.doi.org/10.1094/PDIS-04-19-0773-RE
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