MUR1-mediated cell-wall fucosylation is required for freezing tolerance in Arabidopsis thaliana

MUR1-mediated cell-wall fucosylation is required for freezing tolerance in Arabidopsis thaliana

© 2019 The Authors New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 224(2019), 4 vom: 23. Dez., Seite 1518-1531
1. Verfasser: Panter, Paige E (VerfasserIn)
Weitere Verfasser: Kent, Olivia, Dale, Maeve, Smith, Sarah J, Skipsey, Mark, Thorlby, Glenn, Cummins, Ian, Ramsay, Nathan, Begum, Rifat A, Sanhueza, Dayan, Fry, Stephen C, Knight, Marc R, Knight, Heather
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana sfr8 Rhamnogalacturonan II boron cell wall freezing tolerance fucose pectin mehr... Arabidopsis Proteins mur1 protein, Arabidopsis rhamnogalacturonan II Fucose 28RYY2IV3F Pectins 89NA02M4RX Boron N9E3X5056Q
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520 |a Forward genetic screens play a key role in the identification of genes contributing to plant stress tolerance. Using a screen for freezing sensitivity, we have identified a novel freezing tolerance gene, SENSITIVE-TO-FREEZING8, in Arabidopsis thaliana. We identified SFR8 using recombination-based mapping and whole-genome sequencing. As SFR8 was predicted to have an effect on cell wall composition, we used GC-MS and polyacrylamide gel electrophoresis to measure cell-wall fucose and boron (B)-dependent dimerization of the cell-wall pectic domain rhamnogalacturonan II (RGII) in planta. After treatments to promote borate-bridging of RGII, we assessed freeze-induced damage in wild-type and sfr8 plants by measuring electrolyte leakage from freeze-thawed leaf discs. We mapped the sfr8 mutation to MUR1, a gene encoding the fucose biosynthetic enzyme GDP-d-mannose-4,6-dehydratase. sfr8 cell walls exhibited low cell-wall fucose levels and reduced RGII bridging. Freezing sensitivity of sfr8 mutants was ameliorated by B supplementation, which can restore RGII dimerization. B transport mutants with reduced RGII dimerization were also freezing-sensitive. Our research identifies a role for the structure and composition of the plant primary cell wall in determining basal plant freezing tolerance and highlights the specific importance of fucosylation, most likely through its effect on the ability of RGII pectin to dimerize 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis thaliana 
650 4 |a sfr8 
650 4 |a Rhamnogalacturonan II 
650 4 |a boron 
650 4 |a cell wall 
650 4 |a freezing tolerance 
650 4 |a fucose 
650 4 |a pectin 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a mur1 protein, Arabidopsis  |2 NLM 
650 7 |a rhamnogalacturonan II  |2 NLM 
650 7 |a Fucose  |2 NLM 
650 7 |a 28RYY2IV3F  |2 NLM 
650 7 |a Pectins  |2 NLM 
650 7 |a 89NA02M4RX  |2 NLM 
650 7 |a Boron  |2 NLM 
650 7 |a N9E3X5056Q  |2 NLM 
700 1 |a Kent, Olivia  |e verfasserin  |4 aut 
700 1 |a Dale, Maeve  |e verfasserin  |4 aut 
700 1 |a Smith, Sarah J  |e verfasserin  |4 aut 
700 1 |a Skipsey, Mark  |e verfasserin  |4 aut 
700 1 |a Thorlby, Glenn  |e verfasserin  |4 aut 
700 1 |a Cummins, Ian  |e verfasserin  |4 aut 
700 1 |a Ramsay, Nathan  |e verfasserin  |4 aut 
700 1 |a Begum, Rifat A  |e verfasserin  |4 aut 
700 1 |a Sanhueza, Dayan  |e verfasserin  |4 aut 
700 1 |a Fry, Stephen C  |e verfasserin  |4 aut 
700 1 |a Knight, Marc R  |e verfasserin  |4 aut 
700 1 |a Knight, Heather  |e verfasserin  |4 aut 
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