Small Grain and Dwarf 2, encoding an HD-Zip II family transcription factor, regulates plant development by modulating gibberellin biosynthesis in rice

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 288(2019) vom: 01. Nov., Seite 110208
1. Verfasser:	Chen, Weiwei (VerfasserIn)
Weitere Verfasser:	Cheng, Zhijun, Liu, Linglong, Wang, Min, You, Xiaoman, Wang, Jian, Zhang, Feng, Zhou, Chunlei, Zhang, Zhe, Zhang, Huan, You, Shimin, Wang, Yupeng, Luo, Sheng, Zhang, Jinhui, Wang, Jiulin, Wang, Jie, Zhao, Zhichao, Guo, Xiuping, Lei, Cailin, Zhang, Xin, Lin, Qibing, Ren, Yulong, Zhu, Shanshan, Wan, Jianmin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Dwarfism GA biosynthesis HD-Zip transcription factor Oryza sativa Small grain Gibberellins Plant Growth Regulators Plant Proteins Transcription Factors

Beschreibung
Zusammenfassung:	Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved. Homeodomain leucine zipper (HD-Zip) proteins are transcription factors that regulate plant development. Bioactive gibberellin (GA) is a key endogenous hormone that participates in plant growth. However, the relationship between HD-Zip genes and modulation of GA biosynthesis in rice remains elusive. Here, we identified a rice mutant, designated as small grain and dwarf 2 (sgd2), which had reduced height and grain size compared with the wild type. Cytological observations indicated that the defective phenotype was mainly due to decreased cell length. Map-based cloning and complementation tests demonstrated that a 9 bp deletion in a homeodomain leucine zipper (HD-Zip) II family transcription factor was responsible for the sgd2 mutant phenotype. Expression of SGD2 was pronounced in developing panicles, and its protein was localized in nucleus. Luciferase reporter system and transactivation assays in yeast suggested that SGD2 functioned as a transcriptional repressor. High performance liquid chromatography assays showed that the endogenous GA1 level in the sgd2 mutant was dramatically decreased, and exogenous GA3 recovered the second leaf sheath to normal length. Results of qRT-PCR showed that the expression levels of genes positively regulating GA-biosynthesis were mostly down-regulated in the mutant. Our data identified the role of an HD-Zip transcription factor that affects rice plant development by modulating gibberellin biosynthesis
Beschreibung:	Date Completed 26.12.2019 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2019.110208