Photoexcited CRY1 and phyB interact directly with ARF6 and ARF8 to regulate their DNA-binding activity and auxin-induced hypocotyl elongation in Arabidopsis

Détails bibliographiques
Publié dans:	The New phytologist. - 1979. - 225(2020), 2 vom: 03. Jan., Seite 848-865
Auteur principal:	Mao, Zhilei (Auteur)
Autres auteurs:	He, Shengbo, Xu, Feng, Wei, Xuxu, Jiang, Lu, Liu, Yao, Wang, Wenxiu, Li, Ting, Xu, Pengbo, Du, Shasha, Li, Ling, Lian, Hongli, Guo, Tongtong, Yang, Hong-Quan
Format:	Article en ligne
Langue:	English
Publié:	2020
Accès à la collection:	The New phytologist
Sujets:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Auxin Response Factor auxin blue light cryptochrome 1 hypocotyl elongation phytochrome B red light plus... Arabidopsis Proteins CRY1 protein, Arabidopsis Cryptochromes DNA, Plant DNA-Binding Proteins Indoleacetic Acids PHYB protein, Arabidopsis Transcription Factors auxin response factor 6, Arabidopsis auxin response factor 8, Arabidopsis Phytochrome B 136250-22-1

Description
Résumé:	© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. Arabidopsis CRY1 and phyB are the primary blue and red light photoreceptors mediating blue and red light inhibition of hypocotyl elongation, respectively. Auxin is a pivotal phytohormone involved in promoting hypocotyl elongation. CRY1 and phyB interact with and stabilize auxin/indole acetic acid proteins (Aux/IAAs) to inhibit auxin signaling. The present study investigated whether photoreceptors might interact directly with Auxin Response Factors (ARFs) to regulate auxin signaling. Protein-protein interaction studies demonstrated that CRY1 and phyB interact physically with ARF6 and ARF8 through their N-terminal domains in a blue and red light-dependent manner, respectively. Moreover, the N-terminal DNA-binding domain of ARF6 and ARF8 is involved in mediating their interactions with CRY1. Genetic studies showed that ARF6 and ARF8 act partially downstream from CRY1 and PHYB to regulate hypocotyl elongation under blue and red light, respectively. Chromatin immunoprecipitation-PCR assays demonstrated that CRY1 and phyB mediate blue and red light repression of the DNA-binding activity of ARF6 and ARF6-target gene expression, respectively. Altogether, the results herein suggest that the direct repression of auxin-responsive gene expression mediated by the interactions of CRY1 and phyB with ARFs constitutes a new layer of the regulatory mechanisms by which light inhibits auxin-induced hypocotyl elongation
Description:	Date Completed 01.12.2020 Date Revised 01.12.2020 published: Print-Electronic GENBANK: GSE58552, GSE101873, GSE101875 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.16194