The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls

The plant WEE1 kinase is involved in checkpoint control activation in nematode-induced galls

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 225(2020), 1 vom: 01. Jan., Seite 430-447
1. Verfasser: Cabral, Danila (VerfasserIn)
Weitere Verfasser: Banora, Mohamed Youssef, Antonino, José Dijair, Rodiuc, Natalia, Vieira, Paulo, Coelho, Roberta R, Chevalier, Christian, Eekhout, Thomas, Engler, Gilbert, De Veylder, Lieven, Grossi-de-Sa, Maria Fatima, de Almeida Engler, Janice
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis thaliana Solanum lycopersicum WEE1 kinase cell cycle checkpoint control galls root-knot nematode Arabidopsis Proteins mehr... RNA, Messenger Protein Serine-Threonine Kinases EC 2.7.11.1 WEE1 protein, Arabidopsis Glucuronidase EC 3.2.1.31
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520 |a Galls induced by plant-parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such a background, potential DNA damage in the genome of gall cells is evident. We investigated whether DNA damage checkpoint activation followed by DNA repair occurred, or was eventually circumvented, in nematode-induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1-knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1, were used to understand the mechanisms for coping with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely, disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted at the accumulation of mitotic defects. In addition, WEE1-knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root-knot nematodes. Together with data on DNA-damaging drugs, we suggest a conserved function for WEE1 in controlling G1/S cell cycle arrest in response to a replication defect in galls 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Arabidopsis thaliana 
650 4 |a Solanum lycopersicum 
650 4 |a WEE1 kinase 
650 4 |a cell cycle 
650 4 |a checkpoint control 
650 4 |a galls 
650 4 |a root-knot nematode 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
650 7 |a Protein Serine-Threonine Kinases  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
650 7 |a WEE1 protein, Arabidopsis  |2 NLM 
650 7 |a EC 2.7.11.1  |2 NLM 
650 7 |a Glucuronidase  |2 NLM 
650 7 |a EC 3.2.1.31  |2 NLM 
700 1 |a Banora, Mohamed Youssef  |e verfasserin  |4 aut 
700 1 |a Antonino, José Dijair  |e verfasserin  |4 aut 
700 1 |a Rodiuc, Natalia  |e verfasserin  |4 aut 
700 1 |a Vieira, Paulo  |e verfasserin  |4 aut 
700 1 |a Coelho, Roberta R  |e verfasserin  |4 aut 
700 1 |a Chevalier, Christian  |e verfasserin  |4 aut 
700 1 |a Eekhout, Thomas  |e verfasserin  |4 aut 
700 1 |a Engler, Gilbert  |e verfasserin  |4 aut 
700 1 |a De Veylder, Lieven  |e verfasserin  |4 aut 
700 1 |a Grossi-de-Sa, Maria Fatima  |e verfasserin  |4 aut 
700 1 |a de Almeida Engler, Janice  |e verfasserin  |4 aut 
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773 1 8 |g volume:225  |g year:2020  |g number:1  |g day:01  |g month:01  |g pages:430-447 
856 4 0 |u http://dx.doi.org/10.1111/nph.16185  |3 Volltext 
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