|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM301006466 |
| 003 |
DE-627 |
| 005 |
20231225103730.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2019.08.029
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1003.xml
|
| 035 |
|
|
|a (DE-627)NLM301006466
|
| 035 |
|
|
|a (NLM)31491701
|
| 035 |
|
|
|a (PII)S0981-9428(19)30335-3
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Almeida, Felipe Astolpho
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a LED lamps enhance somatic embryo maturation in association with the differential accumulation of proteins in the Carica papaya L. 'Golden' embryogenic callus
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 03.02.2020
|
| 500 |
|
|
|a Date Revised 30.09.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a The use of light-emitting diode (LED) lamps has been shown to be a promising approach for improving somatic embryo maturation during somatic embryogenesis. The aim of this work was to study the influence of the light source on somatic embryo differentiation and its relationship with the differential abundance of proteins in the Carica papaya L. 'Golden' embryogenic callus at 14 days of maturation. The white plus medium-blue (WmB) LED and fluorescent lamp treatments produced an average of 82.4 and 47.6 cotyledonary somatic embryos per callus, respectively. A shotgun proteomics analysis revealed 28 upaccumulated and 7 downaccumulated proteins. The proteins upaccumulated in the embryogenic callus matured under the WmB LED lamp compared with that matured under the fluorescent lamp included indole-3-acetic acid-amido synthetase (GH3) and actin-depolymerizing factor 2 (ADF2), which are involved in the regulation of auxin levels by auxin conjugation and transport. Additionally, proteins related to energy production (aconitate, ADH1, GAPCp, PKp and TPI), cell wall remodeling (PG and GLPs), and intracellular trafficking (NUP50A, IST1, small GTPases and H+-PPase) showed significantly higher abundance in the embryogenic callus incubated under the WmB LED lamp than in that incubated under the fluorescent lamp. The results showed that the WmB LED lamp improved somatic embryo maturation in association with the differential accumulation of proteins in the C. papaya 'Golden' embryogenic callus
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Carica papaya L.
|
| 650 |
|
4 |
|a Clonal propagation
|
| 650 |
|
4 |
|a Comparative proteomics
|
| 650 |
|
4 |
|a LED
|
| 650 |
|
4 |
|a Label-free proteomics
|
| 650 |
|
4 |
|a Light-emitting diode
|
| 650 |
|
4 |
|a Somatic embryogenesis
|
| 650 |
|
7 |
|a Indoleacetic Acids
|2 NLM
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 700 |
1 |
|
|a Vale, Ellen Moura
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Reis, Ricardo Souza
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Santa-Catarina, Claudete
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Silveira, Vanildo
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 143(2019) vom: 01. Okt., Seite 109-118
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:143
|g year:2019
|g day:01
|g month:10
|g pages:109-118
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2019.08.029
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 143
|j 2019
|b 01
|c 10
|h 109-118
|