Natural variation at OsCERK1 regulates arbuscular mycorrhizal symbiosis in rice

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 225(2020), 4 vom: 03. Feb., Seite 1762-1776
1. Verfasser:	Huang, Renliang (VerfasserIn)
Weitere Verfasser:	Li, Zheng, Mao, Cui, Zhang, Hui, Sun, Zhongfeng, Li, Hao, Huang, Congcong, Feng, Yong, Shen, Xianhua, Bucher, Marcel, Zhang, Zhongming, Lin, Yongjun, Cao, Yangrong, Duanmu, Deqiang
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't CERK1 arbuscular mycorrhizal fungi natural variation rice symbiosis Plant Proteins

Beschreibung
Zusammenfassung:	© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. The symbiotic interaction between arbuscular mycorrhizal fungi (AMF) and land plants is essential for efficient nutrient acquisition and utilisation. Our understanding of key processes controlling the AMF colonisation in rice is still limited. Dongxiang wild rice (DY) exhibited a stronger colonisation with Rhizophagus irregularis than the rice cultivar Zhongzao 35 (ZZ35). Chromosome segment substitution lines were constructed and the OsCERK1 gene from DY was mapped. Transgenic plants in the japonica rice Zhonghua 11 (ZZ11) were constructed to compare root colonisation by AMF. Chromosome single-segment substitution lines containing OsCERK1DY showed higher phosphorus content and grain yield relative to ZZ35. Four amino acids substitutions were identified among the OsCERK1 haplotypes of DY, ZZ35 and ZH11 and two of these were in the second lysine-motif domain, which is essential for the differences of AMF colonisation level among rice varieties. Heterologous expression of OsCERK1DY in ZH11 significantly enhanced AMF colonisation and increased resistance against the pathogenic fungi Magnaporthe oryzae. Notably, the OsCERK1DY haplotype was absent from 4660 cultivated rice varieties. We conclude that OsCERK1 is a key gene affecting the symbiotic interaction with AMF and OsCERK1DY has the biotechnological potential to increase rice phosphorus acquisition and utilisation efficiency for sustainable agriculture
Beschreibung:	Date Completed 08.04.2021 Date Revised 08.04.2021 published: Print-Electronic CommentIn: New Phytol. 2020 Feb;225(4):1404-1406. - PMID 31823373 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.16158