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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1270/jsbbs.18107
|2 doi
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|a pubmed25n1002.xml
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Huang, Bingyan
|e verfasserin
|4 aut
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| 245 |
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|a Marker-assisted backcrossing to improve seed oleic acid content in four elite and popular peanut (Arachis hypogaea L.) cultivars with high oil content
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a High oleic acid composition is an important determinant of seed quality in peanut (Arachis hypogaea) in regard to its nutritional benefits for human health and prolonged shelf-life for peanut products. To improve the oleic acid content of popular peanut cultivars in China, four peanut cultivars of different market types were hybridized with high-oleic-acid donors and backcrossed for four generations as recurrent parents using fad2 marker-assisted backcross selection. Seed quality traits in advanced generations derived by selfing were assessed using near-infrared reflectance spectroscopy for detection of oleic acid and Kompetitive allele-specific PCR (KASP) screening of fad2 mutant markers. Twenty-four high-oleic-acid lines of BC4F4 and BC4F5 populations, with morphological features and agronomic traits similar to those of the recurrent parents, were obtained within 5 years. The genetic backgrounds of BC4F5 lines were estimated using the KASP assay, which revealed the genetic background recovery rate was 79.49%-92.31%. The superior lines raised are undergoing a multi-location test for cultivar registration and release. To our knowledge, this is the first application of single nucleotide polymorphism markers based on the high-throughput and cost-effective KASP assay for detection of fad2 mutations and genetic background evaluation in a peanut breeding program
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4 |
|a Journal Article
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|a Arachis hypogaea
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4 |
|a genetic background recovery
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| 650 |
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|a high oleic acid
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|a marker-assisted backcross selection
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| 650 |
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|a oil content
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|a peanut
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| 650 |
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|a single nucleotide polymorphism
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| 700 |
1 |
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|a Qi, Feiyan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Ziqi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Miao, Lijuan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Zhongxin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Yuanjin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dong, Wenzhao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Fengshou
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zheng, Zheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xinyou
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Breeding science
|d 1998
|g 69(2019), 2 vom: 26. Juni, Seite 234-243
|w (DE-627)NLM098238299
|x 1344-7610
|7 nnns
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| 773 |
1 |
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|g volume:69
|g year:2019
|g number:2
|g day:26
|g month:06
|g pages:234-243
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|u http://dx.doi.org/10.1270/jsbbs.18107
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|h 234-243
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