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231225s2019 xx |||||o 00| ||eng c |
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|a 10.5713/ajas.19.0162
|2 doi
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|a pubmed25n1002.xml
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|a (DE-627)NLM300893329
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|a (NLM)31480175
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Park, Kyu-Mi
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The use of pituitary adenylate cyclase-activating polypeptide in the pre-maturation system improves in vitro developmental competence from small follicles of porcine oocytes
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|c 2019
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Revised 01.10.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a OBJECTIVE: We investigated how pituitary adenylate cyclase-activating polypeptide (PACAP) affects embryonic development during pre-in vitro maturation (pre-IVM) using porcine oocytes isolated from small follicles
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|a METHODS: We divided the follicles into the experimental groups by size (SF, small follicles; MF, medium follicles) and treated with and without PACAP and cultured for 18 hours (Pre-SF[-]PACAP; without PACAP, Pre-SF[+]PACAP; with PACAP) before undergoing IVM. The gene expression related to extracellular matrix formation (amphiregulin, epiregulin, and hyaluronan synthase 2 [HAS2]) and apoptosis (Bcl-2-associated X [BAX], B-cell lymphoma 2, and cysteine-aspartic acid protease 3) was investigated after maturation. The impact on developmental competence was assessed by the cleavage and blastocyst rate and total cell number of blastocysts in embryos generated from parthenogenesis (PA) and in vitro fertilization (IVF)
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| 520 |
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|a RESULTS: Cleavage rates in the Pre-SF(+)PACAP after PA were significantly higher than SF and Pre-SF(-)PACAP (p<0.05). The cleavage rates between MF and Pre- SF(+)PACAP groups yielded no notable differences after IVF. Pre-SF(+)PACAP displayed the higher rate of blastocyst formation and greater total cell number than SF and Pre-SF(-)PACAP (p<0.05). Cumulus cells showed significant upregulation of HAS2 mRNA in the Pre-SF(+)PACAP compared to the SF (p<0.05). In comparison to other groups, the Pre-SF(+)PACAP group displayed a downregulation in mRNA expression of BAX in matured oocytes (p<0.05)
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|a CONCLUSION: The PACAP treatment during pre-IVM improved the developmental potential of porcine oocytes derived from SF by regulating cumulus expansion and apoptosis of oocytes
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4 |
|a Journal Article
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|a Apoptosis
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| 650 |
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4 |
|a Embryo Development
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| 650 |
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4 |
|a Follicles
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| 650 |
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4 |
|a Oocyte Maturation
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| 650 |
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4 |
|a Porcine
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| 700 |
1 |
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|a Kim, Kyu-Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jin, Minghui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Han, Yongquan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a So, Kyoung-Ha
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hyun, Sang-Hwan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Asian-Australasian journal of animal sciences
|d 1998
|g 32(2019), 12 vom: 03. Dez., Seite 1844-1853
|w (DE-627)NLM098195883
|x 1011-2367
|7 nnns
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| 773 |
1 |
8 |
|g volume:32
|g year:2019
|g number:12
|g day:03
|g month:12
|g pages:1844-1853
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| 856 |
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|u http://dx.doi.org/10.5713/ajas.19.0162
|3 Volltext
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|d 32
|j 2019
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|c 12
|h 1844-1853
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