Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

OBJECTIVE: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein s...

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Veröffentlicht in:Asian-Australasian journal of animal sciences. - 1998. - 33(2020), 6 vom: 10. Juni, Seite 1012-1022
1. Verfasser: Liao, Xian-Dong (VerfasserIn)
Weitere Verfasser: Zhou, Chang-Hai, Zhang, Jing, Shen, Jing-Lin, Wang, Ya-Jing, Jin, Yong-Cheng, Li, Sheng-Li
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:Asian-Australasian journal of animal sciences
Schlagworte:Journal Article All-trans Retinoic Acid Casein Fatty Acid MAC-T Cells mRNA Expression
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520 |a OBJECTIVE: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells 
520 |a METHODS: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively 
520 |a RESULTS: In MAC-T cells, ATRA increased the mRNA levels of αS1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA 
520 |a CONCLUSION: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes 
650 4 |a Journal Article 
650 4 |a All-trans Retinoic Acid 
650 4 |a Casein 
650 4 |a Fatty Acid 
650 4 |a MAC-T Cells 
650 4 |a mRNA Expression 
700 1 |a Zhou, Chang-Hai  |e verfasserin  |4 aut 
700 1 |a Zhang, Jing  |e verfasserin  |4 aut 
700 1 |a Shen, Jing-Lin  |e verfasserin  |4 aut 
700 1 |a Wang, Ya-Jing  |e verfasserin  |4 aut 
700 1 |a Jin, Yong-Cheng  |e verfasserin  |4 aut 
700 1 |a Li, Sheng-Li  |e verfasserin  |4 aut 
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