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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12767
|2 doi
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|a pubmed25n1002.xml
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|a (DE-627)NLM300874243
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|a (NLM)31478220
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Rout, Emily D
|e verfasserin
|4 aut
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|a Assessment of immunoglobulin heavy chain, immunoglobulin light chain, and T-cell receptor clonality testing in the diagnosis of feline lymphoid neoplasia
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.03.2020
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|a Date Revised 10.03.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2019 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Differentiation between neoplastic and reactive lymphocytic proliferations can be challenging in cats. PCR for antigen receptor rearrangements (PARR) testing is a useful diagnostic tool to assess clonality of a lymphoid population. Previous feline PARR studies evaluated clonality of complete immunoglobulin heavy chain V-D-J (IGH-VDJ) and T-cell receptor gamma (TRG) gene rearrangements
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|a OBJECTIVES: We aimed to evaluate the sensitivity and specificity of feline PARR primers targeting complete IGH-VDJ and TRG rearrangements, as well as incomplete IGH-DJ, kappa deleting element (Kde), and immunoglobulin lambda light chain (IGL) gene rearrangements in defined feline neoplasms and nonneoplastic controls
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|a METHODS: Fluorescently labeled PCR primers were designed to amplify complete IGH-VDJ, incomplete IGH-DJ, Kde, IGL, and TRG gene rearrangements in two multiplexed PCR reactions, and PCR products were analyzed by fragment analysis. Fresh tissue samples from 12 flow cytometrically confirmed B-cell lymphomas, 26 cytologically confirmed gastric and renal lymphomas of presumed B-cell origin, 30 flow cytometrically confirmed T-cell leukemias, and 11 negative control cats were tested
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|a RESULTS: Using four immunoglobulin primer sets (IGH-VDJ, IGH-DJ, Kde, and IGL), clonal immunoglobulin rearrangements were detected in 87% (33/38) of the presumed B-cell neoplasms. The IGH-VDJ reaction alone only detected clonality in 50% (19/38) of these cases. TRG rearrangements were clonal in 97% (29/30) of the T-cell leukemia cases. All negative control samples had polyclonal immunoglobulin and TRG rearrangements
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|a CONCLUSIONS: The PARR assay developed in this study is useful for assessing clonality in feline lymphoid neoplasms. Clonality assessment of incomplete IGH-DJ, Kde, and IGL rearrangements helped identify clonal B-cell neoplasms not detected with complete IGH-VDJ PARR alone
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|a Journal Article
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|a T-cell receptor gamma
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|a antigen receptor rearrangement
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|a lymphoma
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| 650 |
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|a Immunoglobulin Heavy Chains
|2 NLM
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| 650 |
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|a Immunoglobulin Light Chains
|2 NLM
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| 650 |
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|a Receptors, Antigen
|2 NLM
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| 700 |
1 |
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|a Burnett, Robert C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yoshimoto, Janna A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avery, Paul R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avery, Anne C
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 48 Suppl 1(2019) vom: 21. Okt., Seite 45-58
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
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|g volume:48 Suppl 1
|g year:2019
|g day:21
|g month:10
|g pages:45-58
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| 856 |
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|u http://dx.doi.org/10.1111/vcp.12767
|3 Volltext
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|a AR
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|d 48 Suppl 1
|j 2019
|b 21
|c 10
|h 45-58
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