Dominant inhibition of awn development by a putative zinc-finger transcriptional repressor expressed at the B1 locus in wheat

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 225(2020), 1 vom: 15. Jan., Seite 340-355
1. Verfasser:	Huang, Daiqing (VerfasserIn)
Weitere Verfasser:	Zheng, Qian, Melchkart, Tancey, Bekkaoui, Yasmina, Konkin, David J F, Kagale, Sateesh, Martucci, Martial, You, Frank M, Clarke, Martha, Adamski, Nikolai M, Chinoy, Catherine, Steed, Andrew, McCartney, Curt A, Cutler, Adrian J, Nicholson, Paul, Feurtado, J Allan
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2020
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't B1 locus C2H2 EAR motif awn awnletted transcriptional repression wheat zinc finger mehr... Indoleacetic Acids Plant Proteins Repressor Proteins

Beschreibung
Zusammenfassung:	© 2019 National Research Council Canada New Phytologist © 2019 New Phytologist Trust. Awns, bristle-like structures extending from grass lemmas, provide protection against predators, contribute to photosynthesis and aid in grain dispersal. In wheat, selection of awns with minimal extension, termed awnletted, has occurred during domestication by way of loci that dominantly inhibit awn development, such as Tipped1 (B1), Tipped2 (B2), and Hooded (Hd). Here we identify and characterize the B1 gene. B1 was identified using bulked segregant RNA-sequencing of an F2 durum wheat population and through deletion mapping of awned bread wheat mutants. Functional characterization was accomplished by gene overexpression while haplotype analyses assessed B1 polymorphisms and genetic variation. Located on chromosome 5A, B1 is a C2H2 zinc finger encoding gene with ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs. Constitutive overexpression of B1 in awned wheat produced an awnletted phenotype with pleiotropic effects on plant height and fertility. Transcriptome analysis of B1 overexpression plants suggests a role as transcriptional repressor, putatively targeting pathways involved in cell proliferation. Haplotype analysis revealed a conserved B1 coding region with proximal polymorphisms and supported the contention that B1 is mainly responsible for awnletted wheats globally. B1, predominantly responsible for awn inhibition in wheat, encodes a C2H2 zinc finger protein with EAR motifs which putatively functions as a transcriptional repressor
Beschreibung:	Date Completed 11.11.2020 Date Revised 11.11.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.16154