A streamlined protocol for the detection of mRNA-sRNA interactions using AMT-crosslinking in vitro
Until recently, RNA-RNA interactions were mainly identified by crosslinking RNAs with interacting proteins, RNA proximity ligation and deep sequencing. Recently, AMT-based direct RNA crosslinking was established. Yet, several steps of these procedures are rather inefficient, reducing the output of i...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 67(2019), 4 vom: 29. Okt., Seite 178-183
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| 1. Verfasser: |
Kirsch, Rebecca
(VerfasserIn) |
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| Weitere Verfasser: |
Olzog, V Janett,
Bonin, Sonja,
Weinberg, Christina E,
Betat, Heike,
Stadler, Peter F,
Mörl, Mario |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2019
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Arabidopsis thaliana tRNA ligase (AtRNL)
M-MuLV reverse transcriptase
RNA
RevertAid transcriptase
tRNA 2′-phosphotransferase 1 (Tpt1)
Arabidopsis Proteins
Cross-Linking Reagents
Phosphates
mehr...
RNA, Messenger
RNA Ligase (ATP)
EC 6.5.1.3 |
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