|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM300582846 |
| 003 |
DE-627 |
| 005 |
20250225210144.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231225s2019 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1021/acs.langmuir.9b01979
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n1001.xml
|
| 035 |
|
|
|a (DE-627)NLM300582846
|
| 035 |
|
|
|a (NLM)31448614
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Aybeke, Ece Neslihan
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Nanoscale Mapping of the Physical Surface Properties of Human Buccal Cells and Changes Induced by Saliva
|
| 264 |
|
1 |
|c 2019
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 31.08.2020
|
| 500 |
|
|
|a Date Revised 31.08.2020
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The mucosal pellicle, also called salivary pellicle, is a thin biological layer made of salivary and epithelial constituents, lining oral mucosae. It contributes to their protection against microbiological, chemical, or mechanical insults. Pellicle formation depends on the cells' surface properties, and in turn the pellicle deeply modifies such properties. It has been reported that the expression of the transmembrane mucin MUC1 in oral epithelial cells improves the formation of the mucosal pellicle. Here, we describe an approach combining classical and functionalized tip atomic force microscopy and scanning microwave microscopy to characterize how MUC1 induces changes in buccal cells' morphology, hydrophobicity, and electric properties to elucidate the physicochemical mechanisms involved in the enhancement of the anchoring of salivary proteins. We show that MUC1 expression did not modify drastically the morphology of the epithelial cells' surface. MUC1 expression, however, resulted in the presence of more hydrophobic and more charged areas at the cell surface. The presence of salivary proteins decreased the highest attractive and repulsive forces recorded between the cell surface and a functionalized hydrophobic atomic force microscopy (AFM) tip, suggesting that the most hydrophobic and charged areas participate in the binding of salivary proteins. The cells' dielectric properties were altered by both MUC1 expression and the presence of a mucosal pellicle. We finally show that in the absence of MUC1, the pellicle appeared as a distinct layer poorly interacting with the cells' surface. This integrative AFM/scanning microwave microscopy approach may usefully describe the surface properties of various cell types, with relevance to the bioadhesion or biomimetics fields
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 700 |
1 |
|
|a Ployon, Sarah
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Brulé, Marine
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a De Fonseca, Brice
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Bourillot, Eric
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Morzel, Martine
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Lesniewska, Eric
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Canon, Francis
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 35(2019), 39 vom: 01. Okt., Seite 12647-12655
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:35
|g year:2019
|g number:39
|g day:01
|g month:10
|g pages:12647-12655
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1021/acs.langmuir.9b01979
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 35
|j 2019
|e 39
|b 01
|c 10
|h 12647-12655
|