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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.9b01515
|2 doi
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|a pubmed24n1001.xml
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|a (NLM)31441311
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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1 |
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|a Hämisch, Benjamin
|e verfasserin
|4 aut
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| 245 |
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|a Self-Assembly of Fibrinogen in Aqueous, Thrombin-Free Solutions of Variable Ionic Strengths
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 20.08.2020
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|a Date Revised 20.08.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Fibrinogen not only forms fibrin networks if assisted by thrombin but also exhibits self-assembly in dilute aqueous solutions in the absence of thrombin. It could be shown that self-assembly can be triggered in a controlled way by diluting the ionic strength set to a value of 0.14 M NaCl in the starting solutions. The present work unravels the mechanism of this self-assembly process by means of a combination of time-resolved multiangle static and dynamic light scattering and atomic force microscopy. Analysis was carried out as a function of the ionic strength adjusted by the drop in ionic strength and at variable salt compositions at a given final ionic strength. Composition was varied by changing the ratio of NaCl and phosphate buffer. The self-assembly induced by the drop of the ionic strength depends on the final value. The lower the final ionic strength gets, the faster is the self-assembly process. The variation of the salt composition at a given ionic strength has only a marginal effect, which depends on the ionic strength. The self-assembly obeys a step-growth process, where any intermediate cluster can coalesce with any other cluster. Interpretation of the data with a kinetic model based on the approach of von Smoluchowski follows a diffusion-limited cluster aggregation at ionic strength values lower than 30 mM. At an ionic strength of 30 mM, the model has to take into account a size dependence of the rate constant, and at 60 mM a transition is observed to a reaction-limited cluster aggregation
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|a Journal Article
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|a Solutions
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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|a Fibrinogen
|2 NLM
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|a 9001-32-5
|2 NLM
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| 700 |
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|a Büngeler, Anne
|e verfasserin
|4 aut
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1 |
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|a Kielar, Charlotte
|e verfasserin
|4 aut
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| 700 |
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|a Keller, Adrian
|e verfasserin
|4 aut
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| 700 |
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|a Strube, Oliver
|e verfasserin
|4 aut
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| 700 |
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|a Huber, Klaus
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 35(2019), 37 vom: 17. Sept., Seite 12113-12122
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:35
|g year:2019
|g number:37
|g day:17
|g month:09
|g pages:12113-12122
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|u http://dx.doi.org/10.1021/acs.langmuir.9b01515
|3 Volltext
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