Single-base methylome profiling of the giant kelp Saccharina japonica reveals significant differences in DNA methylation to microalgae and plants

Single-base methylome profiling of the giant kelp Saccharina japonica reveals significant differences in DNA methylation to microalgae and plants

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 225(2020), 1 vom: 01. Jan., Seite 234-249
1. Verfasser: Fan, Xiao (VerfasserIn)
Weitere Verfasser: Han, Wentao, Teng, Linhong, Jiang, Peng, Zhang, Xiaowen, Xu, Dong, Li, Chang, Pellegrini, Matteo, Wu, Chunhui, Wang, Yitao, Kaczurowski, Michelle Joyce Slade, Lin, Xin, Tirichine, Leila, Mock, Thomas, Ye, Naihao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't BS-PCR DNA methylation DNMT2 MeDIP-seq WGBS-seq brown algae gene expression life-cycle stages mehr... Cytosine 8J337D1HZY Oxidoreductases, O-Demethylating EC 1.- Methyltransferases EC 2.1.1.-
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245 1 0 |a Single-base methylome profiling of the giant kelp Saccharina japonica reveals significant differences in DNA methylation to microalgae and plants 
264 1 |c 2020 
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500 |a Date Completed 11.11.2020 
500 |a Date Revised 09.01.2024 
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500 |a Citation Status MEDLINE 
520 |a © 2019 The Authors. New Phytologist © 2019 New Phytologist Trust. 
520 |a Brown algae have convergently evolved plant-like body plans and reproductive cycles, which in plants are controlled by differential DNA methylation. This contribution provides the first single-base methylome profiles of haploid gametophytes and diploid sporophytes of a multicellular alga. Although only c. 1.4% of cytosines in Saccharina japonica were methylated mainly at CHH sites and characterized by 5-methylcytosine (5mC), there were significant differences between life-cycle stages. DNA methyltransferase 2 (DNMT2), known to efficiently catalyze tRNA methylation, is assumed to methylate the genome of S. japonica in the structural context of tRNAs as the genome does not encode any other DNA methyltransferases. Circular and long noncoding RNA genes were the most strongly methylated regulatory elements in S. japonica. Differential expression of genes was negatively correlated with DNA methylation with the highest methylation levels measured in both haploid gametophytes. Hypomethylated and highly expressed genes in diploid sporophytes included genes involved in morphogenesis and halogen metabolism. The data herein provide evidence that cytosine methylation, although occurring at a low level, is significantly contributing to the formation of different life-cycle stages, tissue differentiation and metabolism in brown algae 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a BS-PCR 
650 4 |a DNA methylation 
650 4 |a DNMT2 
650 4 |a MeDIP-seq 
650 4 |a WGBS-seq 
650 4 |a brown algae 
650 4 |a gene expression 
650 4 |a life-cycle stages 
650 7 |a Cytosine  |2 NLM 
650 7 |a 8J337D1HZY  |2 NLM 
650 7 |a Oxidoreductases, O-Demethylating  |2 NLM 
650 7 |a EC 1.-  |2 NLM 
650 7 |a Methyltransferases  |2 NLM 
650 7 |a EC 2.1.1.-  |2 NLM 
700 1 |a Han, Wentao  |e verfasserin  |4 aut 
700 1 |a Teng, Linhong  |e verfasserin  |4 aut 
700 1 |a Jiang, Peng  |e verfasserin  |4 aut 
700 1 |a Zhang, Xiaowen  |e verfasserin  |4 aut 
700 1 |a Xu, Dong  |e verfasserin  |4 aut 
700 1 |a Li, Chang  |e verfasserin  |4 aut 
700 1 |a Pellegrini, Matteo  |e verfasserin  |4 aut 
700 1 |a Wu, Chunhui  |e verfasserin  |4 aut 
700 1 |a Wang, Yitao  |e verfasserin  |4 aut 
700 1 |a Kaczurowski, Michelle Joyce Slade  |e verfasserin  |4 aut 
700 1 |a Lin, Xin  |e verfasserin  |4 aut 
700 1 |a Tirichine, Leila  |e verfasserin  |4 aut 
700 1 |a Mock, Thomas  |e verfasserin  |4 aut 
700 1 |a Ye, Naihao  |e verfasserin  |4 aut 
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