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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12766
|2 doi
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|a pubmed25n1000.xml
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|a (DE-627)NLM300213034
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|a (NLM)31410878
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Harris, Adam Dugger
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Validation and method comparison of the use of densitometry to quantify monoclonal proteins in canine sera
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.03.2020
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|a Date Revised 10.03.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2019 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Densitometric quantitation using serum protein electrophoresis (SPE) is used to monitor monoclonal proteins (M-proteins) in human patients but has not been validated in the dog. Serum globulin concentrations, species-specific radial immunodiffusion (RID), and ELISAs are currently used in veterinary medicine
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|a OBJECTIVE: We aimed to compare four methods that quantify M-proteins using densitometry and biuret protein (dM-protein) measurements. We also validated the best performing method and compared it with the RID and ELISA methods for measuring canine serum M-protein
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| 520 |
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|a METHOD: Serum from six normal dogs and 83 serum samples from 46 dogs with confirmed monoclonal gammopathies were used. A spike and recovery experiment with purified monoclonal IgG and IgM, inter-run and intra-run variability, linearity under dilution, and lower limit of detection were performed. Results of commercial canine RID and ELISA kits for total class-specific immunoglobulin were compared with dM-proteins
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|a RESULTS: The corrected perpendicular drop gating method had <20% error for IgG/γ-globulin and IgM/β-globulin M-protein quantifications. Linearity (r > .99), intra-run CV (1.1%-2.3%), and inter-run CVs (2.0%-3.5%) were acceptable. Correlation between the RID and densitometry results ranged from r = .25 to r = .88, depending on the class. The RID result was greater than that of the biuret total protein in 26/63 (41%) IgA cases. A panel of IgG, IgA, and IgM RIDs failed to correctly identify an IgM paraproteinemia in 6/6 (100%) cases. Densitometry was not comparable with any other tested method
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|a CONCLUSION: Densitometric quantitation is a valid technique for measuring M-proteins in the β- and γ-globulin regions. Immunotyping via RID using the tested kit does not appear to detect IgM. Densitometry is recommended for measuring M-proteins in canine patients
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|a Comparative Study
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|a Journal Article
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|a Validation Study
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|a electrophoresis
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|a immunoglobulin
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4 |
|a monoclonal gammopathy
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| 650 |
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4 |
|a radial immunodiffusion
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| 650 |
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|a Immunoglobulin A
|2 NLM
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| 650 |
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|a Immunoglobulin G
|2 NLM
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| 650 |
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|a Immunoglobulin M
|2 NLM
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| 650 |
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|a Immunoglobulins
|2 NLM
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| 650 |
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|a gamma-Globulins
|2 NLM
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| 700 |
1 |
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|a Rout, Emily
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Avery, Anne
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bolte, Denise
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Belling-Kelly, Erica
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moore, A Russell
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 48 Suppl 1(2019) vom: 30. Okt., Seite 78-87
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnas
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| 773 |
1 |
8 |
|g volume:48 Suppl 1
|g year:2019
|g day:30
|g month:10
|g pages:78-87
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/vcp.12766
|3 Volltext
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 48 Suppl 1
|j 2019
|b 30
|c 10
|h 78-87
|