Cell type-specific gene expression underpins remodelling of cell wall pectin in exocarp and cortex during apple fruit development

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 70(2019), 21 vom: 18. Nov., Seite 6085-6099
1. Verfasser:	Collins, Patrick P (VerfasserIn)
Weitere Verfasser:	O'donoghue, Erin M, Rebstock, Ria, Tiffin, Heather R, Sutherland, Paul W, Schröder, Roswitha, McAtee, Peter A, Prakash, Roneel, Ireland, Hilary S, Johnston, Jason W, Atkinson, Ross G, Schaffer, Robert J, Hallett, Ian C, Brummell, David A
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabinan arabinofuranosidase cell wall epidermis galactan galactosidase hypodermis pectin mehr... rhamnogalacturonan-I Epitopes Galactans Polysaccharides rhamnogalacturonan I araban 11078-27-6 Pectins 89NA02M4RX

Beschreibung
Zusammenfassung:	© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. In apple (Malus×domestica) fruit, the different layers of the exocarp (cuticle, epidermis, and hypodermis) protect and maintain fruit integrity, and resist the turgor-driven expansion of the underlying thin-walled cortical cells during growth. Using in situ immunolocalization and size exclusion epitope detection chromatography, distinct cell type differences in cell wall composition in the exocarp were revealed during apple fruit development. Epidermal cell walls lacked pectic (1→4)-β-d-galactan (associated with rigidity), whereas linear (1→5)-α-l-arabinan (associated with flexibility) was exclusively present in the epidermal cell walls in expanding fruit and then appeared in all cell types during ripening. Branched (1→5)-α-l-arabinan was uniformly distributed between cell types. Laser capture microdissection and RNA sequencing (RNA-seq) were used to explore transcriptomic differences controlling cell type-specific wall modification. The RNA-seq data indicate that the control of cell wall composition is achieved through cell-specific gene expression of hydrolases. In epidermal cells, this results in the degradation of galactan side chains by possibly five β-galactosidases (BGAL2, BGAL7, BGAL10, BGAL11, and BGAL103) and debranching of arabinans by α-arabinofuranosidases AF1 and AF2. Together, these results demonstrate that flexibility and rigidity of the different cell layers in apple fruit during development and ripening are determined, at least in part, by the control of cell wall pectin remodelling
Beschreibung:	Date Completed 10.08.2020 Date Revised 10.08.2020 published: Print Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/erz370