Real-Time Quantification of Cell Internalization Kinetics by Functionalized Bioluminescent Nanoprobes

Real-Time Quantification of Cell Internalization Kinetics by Functionalized Bioluminescent Nanoprobes

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 31(2019), 39 vom: 28. Sept., Seite e1902469
1. Verfasser: Wu, Di (VerfasserIn)
Weitere Verfasser: Yang, Yilong, Xu, Pengcheng, Xu, Duo, Liu, Yang, Castillo, Roxanne, Yan, Ran, Ren, Jie, Zhou, George, Liu, Chaoyong, Qin, Meng, Du, Juanjuan, Hou, Lihua, Chen, Irvin, Kang, Chunsheng, Jin, Lihua, Wen, Jing, Chen, Wei, Lu, Yunfeng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article bioluminescence cell internalization kinetics firefly luciferase nanocapsules real-time quantification Luminescent Agents Nanocapsules Luciferases, Firefly EC 1.13.12.7
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520 |a Cells transport mass dynamically, crossing cell membranes to maintain metabolism and systemic homeostasis, through which biomolecules are also delivered to cells for gene editing, cell reprograming, therapy, and other purposes. Quantifying the translocation kinetics is fundamentally and clinically essential, but remains limited by fluorescence-based technologies, which are semi-quantitative and only provide kinetics information at cellular level or in discrete time. Herein, a real-time method of quantifying cell internalization kinetics is reported using functionalized firefly-luciferase nanocapsules as the probe. This quantitative assay will facilitate the rational design of delivery vectors and enable high-throughput screening of peptides and other functional molecules, constituting an effective tool for broad applications, including drug development and cancer therapy 
650 4 |a Journal Article 
650 4 |a bioluminescence 
650 4 |a cell internalization kinetics 
650 4 |a firefly luciferase 
650 4 |a nanocapsules 
650 4 |a real-time quantification 
650 7 |a Luminescent Agents  |2 NLM 
650 7 |a Nanocapsules  |2 NLM 
650 7 |a Luciferases, Firefly  |2 NLM 
650 7 |a EC 1.13.12.7  |2 NLM 
700 1 |a Yang, Yilong  |e verfasserin  |4 aut 
700 1 |a Xu, Pengcheng  |e verfasserin  |4 aut 
700 1 |a Xu, Duo  |e verfasserin  |4 aut 
700 1 |a Liu, Yang  |e verfasserin  |4 aut 
700 1 |a Castillo, Roxanne  |e verfasserin  |4 aut 
700 1 |a Yan, Ran  |e verfasserin  |4 aut 
700 1 |a Ren, Jie  |e verfasserin  |4 aut 
700 1 |a Zhou, George  |e verfasserin  |4 aut 
700 1 |a Liu, Chaoyong  |e verfasserin  |4 aut 
700 1 |a Qin, Meng  |e verfasserin  |4 aut 
700 1 |a Du, Juanjuan  |e verfasserin  |4 aut 
700 1 |a Hou, Lihua  |e verfasserin  |4 aut 
700 1 |a Chen, Irvin  |e verfasserin  |4 aut 
700 1 |a Kang, Chunsheng  |e verfasserin  |4 aut 
700 1 |a Jin, Lihua  |e verfasserin  |4 aut 
700 1 |a Wen, Jing  |e verfasserin  |4 aut 
700 1 |a Chen, Wei  |e verfasserin  |4 aut 
700 1 |a Lu, Yunfeng  |e verfasserin  |4 aut 
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773 1 8 |g volume:31  |g year:2019  |g number:39  |g day:28  |g month:09  |g pages:e1902469 
856 4 0 |u http://dx.doi.org/10.1002/adma.201902469  |3 Volltext 
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