Effects of nitrate deficiency on nitrate assimilation and chlorophyll synthesis of detached apple leaves

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 142(2019) vom: 15. Sept., Seite 363-371
1. Verfasser:	Wen, Binbin (VerfasserIn)
Weitere Verfasser:	Li, Chen, Fu, Xiling, Li, Dongmei, Li, Ling, Chen, Xiude, Wu, Hongyu, Cui, Xiaowen, Zhang, Xinhao, Shen, Hongyan, Zhang, Wenqian, Xiao, Wei, Gao, Dongsheng
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2019
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Apple leaf Assimilation enzyme Chlorophyll Gene expression Nitrate nitrogen Nitrates 1406-65-1 Nitrogen N762921K75

Beschreibung
Zusammenfassung:	Copyright © 2019. Published by Elsevier Masson SAS. Nitrogen is one of the most important nutrients for plant growth and development. Nitrate nitrogen (NO3--N) is the main form of nitrogen taken up by plants. Understanding the effects of exogenous NO3--N on nitrogen metabolism at the gene expression and enzyme activity levels during nitrogen assimilation and chlorophyll synthesis is important for increasing nitrogen utilization efficiency. In this study, cell morphology, NO3--N uptake rates, the expression of key genes related to nitrogen assimilation and chlorophyll synthesis and enzyme activity in apple leaves under NO3--N deficiency were investigated. The results showed that the cell morphology of apple leaves was irreversibly deformed due to NO3--N deficiency. NO3--N was absorbed slightly one day after NO3--N deficiency treatment and effluxed after 3 days. The relative expression of genes encoding nitrogen assimilation enzymes and the activity of such enzymes decreased significantly after 1 day of NO3--N deficiency treatment. After treatment for 14 days, gene expression was upregulated, enzyme activity was increased, and NO3--N content was increased. NO3--N deficiency hindered the transformation of 5-aminobilinic acid (ALA) to porphobilinogen (PBG), suggesting a possible route by which NO3--N levels affect chlorophyll synthesis. Collectively, the results indicate that NO3--N deficiency affects enzyme activity by altering the expression of key genes in the nitrogen assimilation pathway, thereby suppressing NO3--N absorption and assimilation. NO3--N deficiency inhibits the synthesis of the chlorophyll precursor PBG, thereby hindering chlorophyll synthesis
Beschreibung:	Date Completed 26.11.2019 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2019.07.007