A rapid cell-based assay for determining poloxamer quality in CHO suspension cell culture

Poloxamers are water-soluble polymers that are widely used in cell culture bioprocessing to protect cells against shearing forces. Use of poor-quality poloxamers may lead to a drastic reduction in cell growth, viabilities and productivities in cell culture-based manufacturing. In order to evaluate p...

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Veröffentlicht in:BioTechniques. - 1993. - 67(2019), 3 vom: 01. Sept., Seite 98-109
1. Verfasser: Narayanappa, Athmaram Thimmasandra (VerfasserIn)
Weitere Verfasser: Mwilu, Sam, Holdread, Stacy, Hammett, Kimesha, Bu, George, Dodson, Elizabeth C, Brooks, James W
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2019
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article CHO cells CMATH assay HPLC assay bacterial adhesion to hydrocarbons pluronic poloxamer poloxamer quality shear protectant Protective Agents mehr... Poloxamer 106392-12-5