Rice black-streaked dwarf virus-encoded P5-1 regulates the ubiquitination activity of SCF E3 ligases and inhibits jasmonate signaling to benefit its infection in rice

Rice black-streaked dwarf virus-encoded P5-1 regulates the ubiquitination activity of SCF E3 ligases and inhibits jasmonate signaling to benefit its infection in rice

© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 225(2020), 2 vom: 15. Jan., Seite 896-912
1. Verfasser: He, Long (VerfasserIn)
Weitere Verfasser: Chen, Xuan, Yang, Jin, Zhang, Tianye, Li, Juan, Zhang, Songbai, Zhong, Kaili, Zhang, Hengmu, Chen, Jianping, Yang, Jian
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2020
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Rice black streaked dwarf virus (RBSDV) SCF complex jasmonic acid pathway rice ubiquitination Cyclopentanes Oxylipins Plant Proteins mehr... Protein Subunits Viral Proteins jasmonic acid 6RI5N05OWW Ubiquitin-Protein Ligases EC 2.3.2.27
Beschreibung
Zusammenfassung:© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
SCF (Skp1/Cullin1/F-box) complexes are key regulators of many cellular processes. Viruses encode specific factors to interfere with or hijack these complexes and ensure their infection in plants. The molecular mechanisms controlling this interference/hijack are currently largely unknown. Here, we present evidence of a novel strategy used by Rice black-streaked dwarf virus (RBSDV) to regulate ubiquitination in rice (Oryza sativa) by interfering in the activity of OsCSN5A. We also show that RBSDV P5-1 specifically affects CSN-mediated deRUBylation of OsCUL1, compromising the integrity of the SCFCOI1 complex. We demonstrate that the expressions of jasmonate (JA) biosynthesis-associated genes are not inhibited, whereas the expressions of JA-responsive genes are down-regulated in transgenic P5-1 plants. More importantly, application of JA to P5-1 transgenic plants did not reduce their susceptibility to RBSDV infection. Our results suggest that P5-1 inhibits the ubiquitination activity of SCF E3 ligases through an interaction with OsCSN5A, and hinders the RUBylation/deRUBylation of CUL1, leading to an inhibition of the JA response pathway and an enhancement of RBSDV infection in rice
Beschreibung:Date Completed 01.12.2020
Date Revised 01.12.2020
published: Print-Electronic
CommentIn: New Phytol. 2020 Jan;225(2):601-603. - PMID 31633812
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.16066