Melting-Temperature-Dependent Interactions of Ergosterol with Unsaturated and Saturated Lipids in Model Membranes
Sterols such as cholesterol (Chol) and ergosterol (Erg) are known to regulate membrane properties in higher eukaryotes and in lower eukaryotes, respectively. To better understand the modulation of membrane properties by Erg, binary lipid membranes composed of Erg and diacylglycerophosphocholine (PC)...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 35(2019), 32 vom: 13. Aug., Seite 10640-10647 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2019
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't |
| Résumé: | Sterols such as cholesterol (Chol) and ergosterol (Erg) are known to regulate membrane properties in higher eukaryotes and in lower eukaryotes, respectively. To better understand the modulation of membrane properties by Erg, binary lipid membranes composed of Erg and diacylglycerophosphocholine (PC) were studied in Langmuir monolayer and bilayer vesicle systems. From the excess area measured by pressure-area isotherms, attractive interactions between Erg and saturated PC were significant above the melting temperature (Tm) of PC. Conversely, repulsive interactions were observed at temperatures below Tm. From the analyses of membrane fluidity and polarity using fluorescence probes, similar trends were observed for bilayer systems where Erg had an ordering effect on saturated PC vesicles in the fluid state. However, Chol had a stronger ordering effect than Erg. In unsaturated PC systems, Erg did not alter membrane ordering. These findings demonstrate that the interaction of Erg with the fluid-state PC lipids will maintain lower-eukaryote membranes in a more ordered state, similar to the effect of cholesterol in higher eukaryotes |
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| Description: | Date Completed 25.06.2020 Date Revised 25.06.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.9b01538 |