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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2019.05.034
|2 doi
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|a pubmed24n0996.xml
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|a (DE-627)NLM298925907
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|a (NLM)31279136
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|a (PII)S0981-9428(19)30234-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Xu, Chen
|e verfasserin
|4 aut
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|a Evaluation, characterization, expression profiling, and functional analysis of DXS and DXR genes of Populus trichocarpa
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.12.2019
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2019 Elsevier Masson SAS. All rights reserved.
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|a 1-Deoxy-D-xylulose-5-phosphate synthasse (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) are key enzymes in terpenoid biosynthesis. DXS catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate (DXP) from pyruvate and D-glyceraldehyde-3-phosphate. DXR catalyzes the formation of 2-C-methyl-D-erythritol 4-phosphate (MEP) from DXP. Previous studies of the DXS and DXR genes have focused on herbs, such as Arabidopsis thaliana, Salvia miltiorrhiza, and Amomum villosum, but few studies have been conducted on woody plants. For that reason, we chose Populus trichocarpa as a model woody plant for investigating the DXS and DXR genes. PtDXS exhibited the highest expression level in leaves and the lowest expression in roots. PtDXR showed maximum expression in young leaves, and the lowest expression in mature leaves. The expression profiles revealed by RT-PCR following different elicitor treatments such as abscisic acid, NaCl, PEG6000, H2O2, and cold stress showed that PtDXS and PtDXR were elicitor-responsive genes. Our results showed that the PtDXS gene exhibited diurnal changes, but PtDXR did not. Moreover, overexpression of PtDXR in transgenic poplars improved tolerance to abiotic and biotic stresses. Those results showed that the PtDXR encoded a functional protein, and widely participates in plant growth and development, stress physiological process
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|a Journal Article
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|a DXR
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|a DXS
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|a Expression
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|a HPLC–MS/MS
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|a Populus trichocarpa
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|a Plant Proteins
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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|a Abscisic Acid
|2 NLM
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|a 72S9A8J5GW
|2 NLM
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| 650 |
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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| 650 |
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|a 1-deoxy-D-xylulose 5-phosphate reductoisomerase
|2 NLM
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| 650 |
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|a EC 1.1.1.267
|2 NLM
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| 650 |
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|a Transferases
|2 NLM
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|a EC 2.-
|2 NLM
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|a deoxyxylulose-5-phosphate synthase
|2 NLM
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|a EC 2.2.1.-
|2 NLM
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|a Aldose-Ketose Isomerases
|2 NLM
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| 650 |
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|a EC 5.3.1.-
|2 NLM
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| 700 |
1 |
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|a Wei, Hui
|e verfasserin
|4 aut
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| 700 |
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|a Movahedi, Ali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Weibo
|e verfasserin
|4 aut
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| 700 |
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|a Ma, Xiaoxing
|e verfasserin
|4 aut
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| 700 |
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|a Li, Dawei
|e verfasserin
|4 aut
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| 700 |
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|a Yin, Tongming
|e verfasserin
|4 aut
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| 700 |
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|a Zhuge, Qiang
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 142(2019) vom: 30. Sept., Seite 94-105
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:142
|g year:2019
|g day:30
|g month:09
|g pages:94-105
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|u http://dx.doi.org/10.1016/j.plaphy.2019.05.034
|3 Volltext
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