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231225s2019 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erz313
|2 doi
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|a pubmed25n0996.xml
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|a (DE-627)NLM29884124X
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|a (NLM)31270545
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Xue, De-Xing
|e verfasserin
|4 aut
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| 245 |
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|a LYK4 is a component of a tripartite chitin receptor complex in Arabidopsis thaliana
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|c 2019
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.08.2020
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|a Date Revised 04.12.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a LysM receptor-like kinases (LYKs) of Arabidopsis thaliana (namely LYK1, LYK4 and LYK5) play a major role in chitin perception and immunity against pathogenic fungi. Chitin-induced heterodimerization of LYK1 and LYK5 has been previously reported, but protein interaction partners of LYK4 have not yet been identified. In this study, by analysing mutants we confirmed a role of LYK4 in chitin perception, and found that the ectodomain of LYK4 homodimerizes and also interacts with the ectodomain of LYK5 in vitro. Pull-down experiments with proteins expressed in protoplasts indicated LYK4-LYK4 and LY4-LYK5 interactions in planta. When protoplasts were treated with chitoheptaose or chitin, a protein complex was immunoprecipitated that appeared to be composed of LYK1, LYK4, and LYK5. Similar experiments with proteins expressed in lyk mutant plants suggested that elicitor treatment induced a physical interaction between LYK1 and LYK5 but not between LYK1 and LYK4. Bimolecular fluorescence complementation experiments substantiated these findings. Overall, our data suggest that LYK4 functions as a LYK5-associated co-receptor or scaffold protein that enhances chitin-induced signaling in Arabidopsis
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis thaliana
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|a chitin signaling
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|a plant defense
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|a protein-protein interactions
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|a receptor complex
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|a receptor-like kinase
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Chitin
|2 NLM
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| 650 |
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|a 1398-61-4
|2 NLM
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| 650 |
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|a AT2G23770 protein, Arabidopsis
|2 NLM
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| 650 |
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7 |
|a EC 2.7.11.1
|2 NLM
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| 650 |
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|a Protein Serine-Threonine Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.1
|2 NLM
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| 700 |
1 |
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|a Li, Chun-Lian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Zhi-Ping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Staehelin, Christian
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 70(2019), 19 vom: 15. Okt., Seite 5507-5516
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:70
|g year:2019
|g number:19
|g day:15
|g month:10
|g pages:5507-5516
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erz313
|3 Volltext
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|d 70
|j 2019
|e 19
|b 15
|c 10
|h 5507-5516
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