Hi-Plex2 : a simple and robust approach to targeted sequencing-based genetic screening
We have previously reported Hi-Plex, a multiplex PCR methodology for building targeted DNA sequencing libraries that offers a low-cost protocol compatible with high-throughput processing. Here, we detail an improved protocol, Hi-Plex2, that more effectively enables the robust construction of small-t...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 67(2019), 3 vom: 14. Sept., Seite 118-122
|
|---|
| 1. Verfasser: |
Hammet, Fleur
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Mahmood, Khalid,
Green, Thomas R,
Nguyen-Dumont, Tu,
Southey, Melissa C,
Buchanan, Daniel D,
Lonie, Andrew,
Nathanson, Katherine L,
Couch, Fergus J,
Pope, Bernard J,
Park, Daniel J |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2019
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Hi-Plex
genetic screening
multiplex PCR
targeted DNA sequencing
variant detection
DNA Primers |
|---|